Terheyden H, Menzel C, Wang H, Springer I N, Rueger D R, Acil Y
Department of Oral and Maxillofacial Surgery, Christian Albrechts University, Kiel, Germany.
Int J Oral Maxillofac Surg. 2004 Mar;33(2):164-72. doi: 10.1054/ijom.2003.0500.
In a previous study vascularized bone grafts were prefabricated with recombinant human osteogenic protein-1 (rhOP-1) using blocks of xenogenic bone mineral (BioOss) as scaffolds. The present study addressed the dosage of rhOP-1 and the combination of an external (mould) and internal scaffold (granular BioOss). In five Göttingen minipigs six prefabrication sites in the latissimus dorsi muscles were randomly assigned to groups a-f. Moulds were prepared by shaping collagen/polylactide membranes in a cylindrical form which was filled with 1g BioOss granules and rhOP-1 (a: 0; b: 50; c, f, e: 250; d: 1000 microg of rhOP-1, a-e: cylinder open to muscle, e cylinder perforated, f: cylinder open to subcutaneous fat). After 6 weeks a dose dependency of bone density (a-d: 0%; 9.4%; 15.8%; 31.1%) and vessel density (a-d: 0.3; 2.4; 7.9; 25.4 counts/view) was observed histomorphometrically. Muscular surrounding was advantageous to subcutaneous tissue. Perforations of the membranes increased vessel density and did not impair bone formation. Bone density decreased in the proximity of the polylactide membranes. The membrane material was too soft and partly collapsed and therefore needs not to be reconsidered. The use of BioOss granules with 1000 microg rhOP-1 per gram proved to be a suitable concept for prefabrication of bone transplants.
在先前的一项研究中，使用异种骨矿物质（BioOss）块作为支架，用重组人骨生成蛋白-1（rhOP-1）预制带血管蒂骨移植体。本研究探讨了rhOP-1的剂量以及外部（模具）和内部支架（颗粒状BioOss）的组合。在5只哥廷根小型猪中，将背阔肌中的6个预制部位随机分为a - f组。通过将胶原/聚丙交酯膜制成圆柱形来制备模具，圆柱体内填充1g BioOss颗粒和rhOP-1（a组：0；b组：50；c、e组：250；d组：1000μg rhOP-1，a - e组：圆柱体向肌肉开放，e组圆柱体有穿孔，f组：圆柱体向皮下脂肪开放）。6周后，通过组织形态计量学观察到骨密度（a - d组：0%；9.4%；15.8%；31.1%）和血管密度（a - d组：0.3；2.4；7.9；25.4个计数/视野）存在剂量依赖性。肌肉周围环境比皮下组织更有利。膜的穿孔增加了血管密度且不损害骨形成。聚丙交酯膜附近的骨密度降低。膜材料太软且部分塌陷，因此无需重新考虑。每克BioOss颗粒使用1000μg rhOP-1被证明是预制骨移植体的合适方案。
