Lee Kea Joo, Kim Hyun, Kim Tae Sik, Park Sun-Hwa, Rhyu Im Joo
Institute of Human Genetics and Department of Anatomy, Division of Brain Korea 21 Biomedical Science, Korea University College of Medicine, 126-1 Anam-Dong 5-Ga, Seongbuk-Ku, Seoul, 136-705, South Korea.
Neurosci Lett. 2004 Apr 8;359(1-2):21-4. doi: 10.1016/j.neulet.2004.01.071.
Morphological changes in spine shapes have been implicated as indications for physiological or pathological status. To investigate the normal distribution ratio of spine shapes of rat Purkinje cells, morphological analysis was conducted using high-voltage electron microscopy following Golgi impregnation. Spines were classified into thin, stubby, mushroom, branched, and unclassified type by their distinct morphological features. In the tertiary branches of Purkinje cell dendrites, proportions of each category were 69.11+/-1.38% (thin), 13.5+/-1.23% (stubby), 10.45+/-0.74% (mushroom), 2.21+/-0.31% (branched), and 4.73+/-0.52% (unclassified). These results suggest that dendritic spines of Purkinje cells may tend to cluster in defined groups by shapes implying that different spine shapes could reflect different functional roles. This classification could be applied for further study of spine plasticity in various conditions.
脊柱形状的形态学变化已被认为是生理或病理状态的指标。为了研究大鼠浦肯野细胞脊柱形状的正常分布比例，在高尔基浸染后使用高压电子显微镜进行了形态学分析。根据其明显的形态特征，脊柱被分为细型、短粗型、蘑菇型、分支型和未分类型。在浦肯野细胞树突的三级分支中，各类别的比例分别为69.11±1.38%（细型）、13.5±1.23%（短粗型）、10.45±0.74%（蘑菇型）、2.21±0.31%（分支型）和4.73±0.52%（未分类型）。这些结果表明，浦肯野细胞的树突棘可能倾向于按形状聚集在特定的组中，这意味着不同的脊柱形状可能反映不同的功能作用。这种分类可用于进一步研究各种条件下的脊柱可塑性。
