Sakai Shinji, Kawabata Kenji, Ono Tsutomu, Ijima Hiroyuki, Kawakami Koei
Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan.
Biotechnol Bioeng. 2004 Apr 20;86(2):168-73. doi: 10.1002/bit.20006.
The droplet breakup technique with an immiscible liquid coflowing stream was investigated for the preparation of mammalian cell-enclosing subsieve-sized capsules of less than 100 microm in diameter. The major parts of the droplet generation device were a needle of several hundred micrometers in diameter for extruding the cell-suspending sodium alginate aqueous solution and a tubule of 2.5 mm in diameter through which the extruded alginate solution flowed into ambient immiscible liquid paraffin. The needle was positioned upstream in the vicinity of the coaxial tubule. The droplet diameter of the viscous sodium alginate aqueous solution could be controlled from several dozen to several hundred micrometers by changing the velocities of the inner and ambient fluids and the diameter of the needle. By utilizing a 300-microm diameter needle, CHO-K1 cell-enclosing droplets of 48 +/- 8 microm in diameter were obtained by extruding a cell-suspending sodium alginate solution at a velocity of 1.2 cm/sec into the ambient liquid paraffin flowing at a velocity of 23.5 cm/sec. The breakup process did not influence the viability of the enclosed cells, since more than 95% of the CHO-K1 cells remained alive after the enclosing process.
研究了采用不混溶液体共流的液滴破碎技术来制备直径小于100微米的包封哺乳动物细胞的亚筛尺寸胶囊。液滴生成装置的主要部分是一根直径几百微米的针，用于挤出悬浮细胞的海藻酸钠水溶液，以及一根直径2.5毫米的小管，挤出的海藻酸钠溶液通过该小管流入周围不混溶的液体石蜡中。针位于同轴小管附近的上游。通过改变内部和周围流体的速度以及针的直径，可以将粘性海藻酸钠水溶液的液滴直径控制在几十到几百微米之间。使用直径300微米的针，以1.2厘米/秒的速度将悬浮细胞的海藻酸钠溶液挤出到以23.5厘米/秒的速度流动的周围液体石蜡中，得到了直径为48±8微米的包封CHO-K1细胞的液滴。破碎过程不影响包封细胞的活力，因为在包封过程后超过95%的CHO-K1细胞仍存活。
