Sakai Shinji, Mu Changjun, Kawabata Kenji, Hashimoto Ichiro, Kawakami Koei
Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Fukuoka 819-0395, Japan.
J Biomed Mater Res A. 2006 Aug;78(2):394-8. doi: 10.1002/jbm.a.30676.
Biocompatibility of cell-enclosing capsules, defined as suppression of pericapsular cellular reactions, is one of the factors governing the success of enclosed cell transplantation in in vivo cell therapy. Agarose capsules of subsieve size, less than 100 microm in diameter, and conventional size, approximately 300-1,000 microm in diameter, were implanted into the peritoneal cavity and epididymal fat pads of mice and rats, respectively, to determine the effect of a reduction in diameter to subsieve size. The degree of cellular reaction to the subsieve-size capsules was much lower than that of the conventional-size microcapsules, independent of implantation site. The frequency of overgrown subsieve-size capsules retrieved from the peritoneal cavities was less than 5% in contrast to approximately 20% for capsules 387 microm in diameter. In addition, no increase in floating cells, which are generated through capsule stimulation, was observed in the peritoneal cavity only with subsieve-size capsules. From these results, we concluded that subsieve-size capsules are more biocompatible than microcapsules of conventional size.
细胞包裹胶囊的生物相容性定义为对囊周细胞反应的抑制，它是体内细胞治疗中决定包裹细胞移植成功与否的因素之一。分别将小于100微米的亚筛尺寸和直径约300 - 1000微米的常规尺寸的琼脂糖胶囊植入小鼠的腹腔和大鼠的附睾脂肪垫，以确定直径减小至亚筛尺寸的影响。与植入部位无关，对亚筛尺寸胶囊的细胞反应程度远低于常规尺寸微胶囊。从腹腔中回收的过度生长的亚筛尺寸胶囊的频率小于5%，而直径387微米的胶囊约为20%。此外，仅使用亚筛尺寸胶囊时，在腹腔中未观察到因胶囊刺激而产生的漂浮细胞增加。从这些结果来看，我们得出结论，亚筛尺寸的胶囊比常规尺寸的微胶囊具有更高的生物相容性。
