Kanda T, Nakatomi Y, Ishikawa H, Hitomi M, Matsubara Y, Ono T, Muto T
Department of Biochemistry, Niigata University School of Medicine, Japan.
Dig Dis Sci. 1992 Sep;37(9):1362-7. doi: 10.1007/BF01296004.
Determination of the serum level of intestinal fatty acid-binding protein has been used to detect rat intestinal ischemia following ligation or 30-min occlusion of the superior mesenteric artery. The normal values were under the minimal detectable level of less than 2 ng/ml in all the 10 rats. The serum fatty acid-binding protein level increased rapidly, to 340.7 +/- 54.6, 438.5 +/- 40.1, 388.1 +/- 37.4, and 292.2 +/- 95.7 ng/ml (P less than 0.01) at 1, 2, 4, and 8 hr after ligation, respectively. It also increased, to 347.2 +/- 127.7 ng/ml (P less than 0.01) at 1 hr, after a 30-min transient occlusion and then returned to a normal level. Histological studies showed destruction of the villi, disappearance of the mucosa, and transmural necrosis with the progress of time after ligation, while no remarkable morphological change was observed following 30-min transient occlusion. These observations strongly suggest that the intestinal fatty acid-binding protein is a useful biochemical marker for intestinal ischemia, particularly in the early reversible phase.
血清肠脂肪酸结合蛋白水平的测定已被用于检测大鼠在肠系膜上动脉结扎或30分钟闭塞后的肠道缺血情况。在所有10只大鼠中，正常值均低于最低可检测水平，即低于2 ng/ml。结扎后1、2、4和8小时，血清脂肪酸结合蛋白水平迅速升高，分别达到340.7±54.6、438.5±40.1、388.1±37.4和292.2±95.7 ng/ml（P<0.01）。在30分钟短暂闭塞后1小时，该水平也升高至347.2±127.7 ng/ml（P<0.01），然后恢复到正常水平。组织学研究显示，结扎后随着时间的推移，绒毛遭到破坏，黏膜消失，出现透壁性坏死，而在30分钟短暂闭塞后未观察到明显的形态学变化。这些观察结果有力地表明，肠脂肪酸结合蛋白是肠道缺血的一种有用的生化标志物，特别是在早期可逆阶段。
