Hazir Selçuk, Stackebrandt Erko, Lang Elke, Schumann Peter, Ehlers Ralf-Udo, Keskin Nevin
Hacettepe University Faculty of Science, Department of Biology, Beytepe, Ankara, Turkey.
Syst Appl Microbiol. 2004 Feb;27(1):36-42. doi: 10.1078/0723-2020-00255.
Bacterial isolates from nematodes from Turkish soil samples were initially characterized by molecular methods and seven members of the genus Photorhabdus identified to the species level, using riboprint analyses and metabolic properties. Strain 07-5 (DSM 15195) was highly related to the type strain of Photorhabdus luminescens subsp. laumondii DSM 15139T, and was regarded a strain of this subspecies. Strains 1121T (DSM 15194T), 68-3 (DSM 15198) and 47-10 (DSM 15197) formed one, strain 39-8T (DSM 15199T), 39-7 (DSM 15196) and 01-12 (DSM 15193) formed a second cluster that branched intermediate the three subspecies of Photorhabdus luminescens. Based upon moderate 16S rRNA gene sequence similarities and differences in metabolic properties among themselves and with type strains of the three subspecies we consider the two clusters to represent two new subspecies of Photorhabdus luminescens for which the names Photorhabdus luminescens subsp. kayaii, type strain 1121T (DSM 15194T, NCIMB 13951T), and Photorhabdus luminescens subsp. thracensis subsp. nov., type strain 39-8T (DSM 15199T, NCIMB 13952T) are proposed.
最初采用分子方法对从土耳其土壤样本中的线虫分离出的细菌进行了鉴定，并通过核糖体印记分析和代谢特性，将光杆状菌属的七个成员鉴定到种水平。菌株07-5（DSM 15195）与发光光杆状菌劳蒙亚种的模式菌株DSM 15139T高度相关，被视为该亚种的一个菌株。菌株1121T（DSM 15194T）、68-3（DSM 15198）和47-10（DSM 15197）形成一个簇，菌株39-8T（DSM 15199T）、39-7（DSM 15196）和01-12（DSM 15193）形成第二个簇，该簇在发光光杆状菌的三个亚种之间分支。基于中等程度的16S rRNA基因序列相似性以及它们自身之间和与三个亚种模式菌株的代谢特性差异，我们认为这两个簇代表发光光杆状菌的两个新亚种，为此提出了发光光杆状菌凯亚亚种，模式菌株1121T（DSM 15194T，NCIMB
13951T），以及发光光杆状菌色雷斯亚种，模式菌株39-8T（DSM 15199T，NCIMB 13952T）。
