Lanoot Benjamin, Vancanneyt Marc, Dawyndt Peter, Cnockaert Margo, Zhang Jianli, Huang Ying, Liu Zhiheng, Swings Jean
BCCM/LMG Bacteria Collection, Ghent University, Belgium.
Syst Appl Microbiol. 2004 Feb;27(1):84-92. doi: 10.1078/0723-2020-00257.
The type strains of 451 validly described Streptomyces species were screened using the BOX-PCR fingerprint technique. Of the different primers tested, the BOX primer yielded the most robust and reproducible band patterns. The majority of the species (350) had an unique pattern each. A total number of 30 clusters were delineated comprising species with nearly identical patterns. Four of these clusters grouped synonymous Streptomyces species known from previous studies. A high correlation exists between BOX-PCR fingerprinting and DNA-DNA homology data. In the present study, the latter was confirmed for 7 additional BOX clusters and led to the following emended descriptions: S. cochleatus is a subjective synonym of S. cinereorectus, S. roseoflavus of S. fradiae, S. roseodiastaticus of S. tricolor, S. roseosporus of S. filamentosus, S. distallicus of S. colombiensis, S. arabicus of S. vinaceus and S. phaeoviridis of S. phaeopurpureus. Although not yet confirmed by DNA-DNA pairing studies, rep-PCR data suggested the presence of at least 33 additional junior synonyms.
使用BOX-PCR指纹技术对451个有效描述的链霉菌物种的模式菌株进行了筛选。在所测试的不同引物中，BOX引物产生的条带模式最为稳定且可重复。大多数物种（350个）各有独特的模式。总共划分出30个簇，每个簇包含模式几乎相同的物种。其中四个簇将先前研究中已知的同义链霉菌物种归为一组。BOX-PCR指纹图谱与DNA-DNA同源性数据之间存在高度相关性。在本研究中，对另外7个BOX簇进行了DNA-DNA同源性验证，并得出以下修订描述：耳蜗链霉菌是灰直链霉菌的主观同义词，玫瑰黄链霉菌是弗氏链霉菌的同义词，玫瑰糖化链霉菌是三色链霉菌的同义词，玫瑰孢链霉菌是丝状链霉菌的同义词，远距链霉菌是哥伦比亚链霉菌的同义词，阿拉伯链霉菌是酒红链霉菌的同义词，以及暗绿链霉菌是暗紫链霉菌的同义词。尽管尚未通过DNA-DNA配对研究得到证实，但rep-PCR数据表明至少还有33个次同义词存在。
