Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia.
Faculty of Medicine and Health Sciences, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia.
Dis Markers. 2018 Aug 1;2018:1351634. doi: 10.1155/2018/1351634. eCollection 2018.
In the last decades, leptospirosis had gained public health concern due to morbidity and mortality rates caused by pathogenic . The need for rapid and robust molecular typing methods to differentiate this zoonotic pathogen is of utmost importance. Various studies had been conducted to determine the genetic relatedness of isolates using molecular typing methods. In this study, 29 pathogenic isolates from rat, soil, and water samples in Sarawak, Malaysia, were characterized using BOX-PCR and ERIC-PCR. The effectiveness of these two methods with regard to the ease of interpretation, reproducibility, typeability, and discriminatory power was also being evaluated. Using BOX-PCR, six clusters and 3 single isolates were defined at a genetic distance percentage of 11.2%. ERIC-PCR clustered the isolates into 6 clusters and 2 single isolates at a genetic distance percentage of 6.8%. Both BOX-PCR and ERIC-PCR produced comparable results though the discriminatory index for ERIC-PCR (0.826) was higher than that for BOX-PCR (0.809). From the constructed dendrogram, it could be summarized that the isolates in this study were highly heterogeneous and genetically diverse. The findings from this study indicated that there is no genetic relatedness among the pathogenic isolates in relation to the locality, source, and identity, with some exceptions. Out of the 29 pathogenic isolates studied, BOX-PCR and ERIC-PCR successfully discriminated 4 isolates (2 isolates each) into the same cluster in relation to sample sources, as well as 2 isolates into the same cluster in association with the sample locality. Future studies shall incorporate the use of other molecular typing methods to make a more thorough comparison on the genetic relatedness of pathogenic .
在过去的几十年中，由于致病性钩端螺旋体引起的发病率和死亡率，钩端螺旋体病引起了公众的健康关注。快速而强大的分子分型方法来区分这种人畜共患病病原体至关重要。已经进行了各种研究，使用分子分型方法来确定分离株的遗传相关性。在这项研究中，使用 BOX-PCR 和 ERIC-PCR 对来自马来西亚沙捞越州大鼠、土壤和水样的 29 株致病性钩端螺旋体分离株进行了特征描述。还评估了这两种方法在解释的容易程度、重现性、分型能力和区分能力方面的有效性。使用 BOX-PCR，在遗传距离百分比为 11.2%的情况下，将 6 个聚类和 3 个单分离株定义为聚类。ERIC-PCR 将分离株聚类为 6 个聚类和 2 个单分离株，遗传距离百分比为 6.8%。BOX-PCR 和 ERIC-PCR 均产生了可比的结果，尽管 ERIC-PCR 的区分指数（0.826）高于 BOX-PCR（0.809）。从构建的系统发育树可以总结出，本研究中的分离株高度异质且遗传多样。研究结果表明，与地理位置、来源和身份无关，致病性钩端螺旋体分离株之间没有遗传相关性，但也有一些例外。在所研究的 29 株致病性钩端螺旋体分离株中，BOX-PCR 和 ERIC-PCR 成功地将 4 株（每株 2 株）分离株根据样品来源和 2 株分离株根据样品来源分为同一聚类。未来的研究应结合使用其他分子分型方法，对致病性钩端螺旋体的遗传相关性进行更全面的比较。
