Uyan Seda Ersus, Baysal Taner, Yurdagel Unal, El Sedef Nehir
Ege University, Engineering Faculty, Food Engineering Department, TK-35100 Bornova-Izmir, Turkey.
Nahrung. 2004 Feb;48(1):57-60. doi: 10.1002/food.200300373.
Purple carrot was analysed for its antioxidant activity by measuring its scavenging effect on 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical. The amount of purple carrot necessary to decrease by 50% the initial DPPH concentration (EC50) was found 30.23 +/- 5.17 (mg sample/mg DPPH). Effects of the dehydration process on the antioxidant activity of purple carrots were also determined after dehydration by using tray drier and microwave+tray drier combination. Purple carrots were cleaned, washed, sorted, sliced, and blanched in water at 98 +/- 1 degree C for 2 min. Then half of the samples were dehydrated in microwave (45 min) and tray drier (105 min) combination, and others were dehydrated in tray drier (150 min) until reaching the constant weight. Raw, blanched and dehydrated purple carrots were compared for their antioxidant activity and total anthocyanin contents, color values (L*, a*, b*, C*, H degrees) and dry matter contents. The antioxidant activity as measured by DPPH, EC50 value ranged from 7.80 to 30.23 of processed and raw carrots where the H degrees values were changed from -3.68 to -23.96 corresponding to a bluish hue.
通过测量紫胡萝卜对1,1-二苯基-2-苦基肼(DPPH)自由基的清除作用来分析其抗氧化活性。发现使初始DPPH浓度降低50%(EC50)所需的紫胡萝卜量为30.23±5.17(毫克样品/毫克DPPH)。在使用托盘干燥器和微波+托盘干燥器组合进行脱水后,还测定了脱水过程对紫胡萝卜抗氧化活性的影响。将紫胡萝卜清洗、冲洗、挑选、切片,并在98±1℃的水中热烫2分钟。然后,一半样品采用微波(45分钟)和托盘干燥器(105分钟)组合进行脱水,另一半样品采用托盘干燥器(150分钟)进行脱水,直至达到恒重。比较了生的、热烫过的和脱水后的紫胡萝卜的抗氧化活性、总花青素含量、颜色值(L*、a*、b*、C*、H°)和干物质含量。通过DPPH测定的抗氧化活性,加工后的胡萝卜和生胡萝卜的EC50值在7.80至30.23之间,其中H°值从-3.68变化到-23.96,对应于蓝色调。