Gülçin Ilhami, Berashvili Dali, Gepdiremen Akçahan
Atatürk University, Faculty of Science and Arts, Department of Chemistry, 25240 Erzurum, Turkey.
J Ethnopharmacol. 2005 Oct 3;101(1-3):287-93. doi: 10.1016/j.jep.2005.05.006.
The purpose of this work comprise the assessment of the antioxidant activity of total anthocyanins from Perilla pankinensis samples (TAPP) by different analytical methods: 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, total antioxidant activity, total reducing activity, superoxide anion radical scavenging, hydrogen peroxide scavenging and metal chelating activities. Total antioxidant activity was measured according to ferric thiocyanate method. alpha-Tocopherol and trolox, which water-soluble analogue of tocopherol were used as references antioxidant compounds. At the concentrations of 30 and 45 microg/mL, TAPP showed 91.9 and 96.4% inhibition on lipid peroxidation of linoleic acid emulsion, respectively. In comparison, 45 microg/mL of standard antioxidant such as alpha-tocopherol and trolox exhibited 88.8 and 86.2% inhibition on peroxidation of linoleic acid emulsion, respectively. In addition, TAPP have found effective on DPPH scavenging, superoxide anion radical and hydrogen peroxide scavenging, total reducing power and metal chelating on ferrous ions activities. Also, these various antioxidant effects were compared to alpha-tocopherol and trolox.
本研究旨在通过不同分析方法评估紫苏总花色苷（TAPP）的抗氧化活性：1,1 - 二苯基 - 2 - 苦基肼（DPPH）自由基清除能力、总抗氧化活性、总还原能力、超氧阴离子自由基清除能力、过氧化氢清除能力及金属螯合活性。总抗氧化活性采用硫氰酸铁法测定。以α - 生育酚及其水溶性类似物Trolox作为抗氧化参考化合物。在30和45μg/mL浓度下，TAPP对亚油酸乳液脂质过氧化的抑制率分别为91.9%和96.4%。相比之下，45μg/mL的标准抗氧化剂如α - 生育酚和Trolox对亚油酸乳液过氧化的抑制率分别为88.8%和86.2%。此外，TAPP在DPPH清除、超氧阴离子自由基和过氧化氢清除、总还原能力以及对亚铁离子的金属螯合活性方面均表现出有效性。同时，将这些不同的抗氧化效果与α - 生育酚和Trolox进行了比较。
