Jacobson Elliott R, Heard Darryl, Andersen Arthur
Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USA.
J Vet Diagn Invest. 2004 Mar;16(2):153-4. doi: 10.1177/104063870401600211.
Tissues were evaluated from emerald tree boas, Corallus caninus, from a collection in which chlamydiosis was diagnosed. To determine the strain of chlamydia infecting these snakes, tissue samples from 5 frozen snakes were tested by a quantitative TaqMan polymerase chain reaction (PCR) test and a PCR sequence analysis test. Of the 22 samples tested, 9 were categorized as either positive or weakly positive with the TaqMan test, and 6 yielded an amplicon using a serial PCR test that amplified a portion of the 23S ribosomal RNA gene. A PCR product suitable for sequencing was obtained from the heart of one of the snakes. Sequence analysis showed that the snake had been infected with Chlamydophila pneumoniae. These findings show that C. pneumoniae can infect emerald tree boas, broadening the range of reptiles known to be infected by this primarily human pathogen.
对来自一批被诊断患有衣原体病的翠绿森蚺（Corallus caninus）的组织进行了评估。为了确定感染这些蛇的衣原体菌株，对5条冷冻蛇的组织样本进行了定量TaqMan聚合酶链反应（PCR）检测和PCR序列分析检测。在检测的22个样本中，9个在TaqMan检测中被归类为阳性或弱阳性，6个通过连续PCR检测产生了扩增子，该检测扩增了23S核糖体RNA基因的一部分。从其中一条蛇的心脏获得了适合测序的PCR产物。序列分析表明，这条蛇感染了肺炎衣原体。这些发现表明，肺炎衣原体可以感染翠绿森蚺，扩大了已知受这种主要感染人类的病原体感染的爬行动物范围。
