Yang Huang-Hao, Zhang Shu-Qiong, Yang Wei, Chen Xiao-Lan, Zhuang Zhi-Xia, Xu Jin-Gou, Wang Xiao-Ru
The First Institute of Oceanography, SOA, Qingdao, P. R. China.
J Am Chem Soc. 2004 Apr 7;126(13):4054-5. doi: 10.1021/ja0389570.
In this study, we report a simple procedure for applying molecular imprinting functional groups to the inner surfaces of the template-synthesized sol-gel nanotubes for chemical separation of estrone. The silica nanotubes were synthesized within the pores of nanopore alumina template membranes using a sol-gel method by simultaneous hydrolysis of a silica monomer-imprinted molecule complex and tetraethoxysilane (TEOS). A covalent imprinting strategy was employed by generating a sacrificial spacer through the reaction of the isocyanate group of 3-(triethoxysilyl)propyl isocyanate and a phenol moiety of estrone to form a thermally cleavable urethane bond. This allowed us to remove the imprinted estrone by simple thermal reaction and to simultaneously introduce functional groups into the cavity formed by the silica nanotubes. Experiments indicated that estrone could be bound selectively by such an approach and have a binding affinity of 864 +/- 137 (n = 3).
在本研究中，我们报告了一种简单的方法，即将分子印迹官能团应用于模板合成的溶胶-凝胶纳米管内表面，用于雌酮的化学分离。通过溶胶-凝胶法，在纳米孔氧化铝模板膜的孔内，使二氧化硅单体-印迹分子复合物与四乙氧基硅烷（TEOS）同时水解，合成了二氧化硅纳米管。采用共价印迹策略，通过3-(三乙氧基硅基)丙基异氰酸酯的异氰酸酯基团与雌酮的酚部分反应生成牺牲间隔基，形成可热裂解的脲键。这使我们能够通过简单的热反应去除印迹的雌酮，并同时将官能团引入由二氧化硅纳米管形成的腔内。实验表明，通过这种方法雌酮能够被选择性结合，结合亲和力为864±137（n = 3）。
