Wang Xin, Wang Lianyan, He Xiwen, Zhang Yukui, Chen Langxing
Department of Chemistry, Nankai University, Tianjin 300071, PR China.
Talanta. 2009 Apr 30;78(2):327-32. doi: 10.1016/j.talanta.2008.11.024. Epub 2008 Nov 27.
In this study, we synthesized Fe(3)O(4) magnetic nanoparticles coated estrone-imprinted polymer with controlled size using a semi-covalent imprinting strategy. In this protocol, the estrone-silica monomer complex (EstSi) was synthesized by the reaction 3-(triethoxysilyl)propyl isocyanate with estrone, where the template was linked to the silica coating on the iron oxide core via a thermally reversible bond. The removal of the template by a simple thermal reaction produced specific estrone recognition sites on the surface of silica shell.The resulting estrone-imprinted polymer coating Fe(3)O(4) magnetic hybrid nanoparticles exhibit a much higher specific recognition and saturation magnetization. The hybrid nanoparticles have been used for biochemical separation of estrone.
在本研究中，我们采用半共价印迹策略合成了尺寸可控的包覆有雌酮印迹聚合物的Fe(3)O(4)磁性纳米粒子。在此方案中，通过3-(三乙氧基硅基)丙基异氰酸酯与雌酮反应合成了雌酮-二氧化硅单体复合物(EstSi)，其中模板通过热可逆键与氧化铁核上的二氧化硅涂层相连。通过简单的热反应去除模板，在二氧化硅壳表面产生了特定的雌酮识别位点。所得的包覆有雌酮印迹聚合物的Fe(3)O(4)磁性杂化纳米粒子表现出更高的特异性识别和饱和磁化强度。该杂化纳米粒子已用于雌酮的生化分离。
