Morier Patrick, Vollet Christine, Michel Philippe E, Reymond Frédéric, Rossier Joël S
DiagnoSwiss SA, Monthey, Switzerland.
Electrophoresis. 2004 Nov;25(21-22):3761-8. doi: 10.1002/elps.200406093.
A way of using gravity flow to induce a linear convection within a microfluidic system is presented. It is shown and mathematically supported that tilting a 1 cm long covered microchannel is enough to generate flow rates up to 1000 nL.min(-1), which represents a linear velocity of 2.4 mm.s(-1). This paper also presents a method to monitor the microfluidic events occurring in a covered microchannel when a difference of pressure is applied to force a solution to flow in said covered microchannel, thanks to electrodes inserted in the microfluidic device. Gravity-induced flow monitored electrochemically is applied to the performance of a parallel-microchannel enzyme-linked immunosorbent assay (ELISA) of the thyroid-stimulating hormone (TSH) with electrochemical detection. A simple method for generating and monitoring fluid flows is described, which can, for instance, be used for controlling parallel assays in microsystems.
本文提出了一种利用重力流在微流控系统中诱导线性对流的方法。结果表明并通过数学证明，倾斜一个1厘米长的覆盖微通道足以产生高达1000 nL·min⁻¹的流速，这相当于2.4 mm·s⁻¹的线性速度。本文还提出了一种方法，当在所述覆盖微通道中施加压力差以迫使溶液流动时，借助插入微流控装置中的电极来监测在覆盖微通道中发生的微流控事件。通过电化学监测的重力诱导流被应用于甲状腺刺激激素（TSH）的平行微通道酶联免疫吸附测定（ELISA）并进行电化学检测。描述了一种产生和监测流体流动的简单方法，例如，该方法可用于控制微系统中的平行测定。
