de Oliveira Débora, Di Luccio Marco, Faccio Carina, Rosa Clarissa Dalla, Bender João Paulo, Lipke Nádia, Menoncin Silvana, Amroginski Cristiana, de Oliveira José Vladimir
Department of Food Engineering, URI, Campus de Erechim, Av. Sete de Setembro, 1621-Erechim-RS, 99700-000, Brazil.
Appl Biochem Biotechnol. 2004 Spring;113-116:771-80. doi: 10.1385/abab:115:1-3:0771.
We studied the production of fatty acid ethyl esters from castor oil using n-hexane as solvent and two commercial lipases, Novozym 435 and Lipozyme IM, as catalysts. For this purpose, a Taguchi experimental design was adopted considering the following variables: temperature (35-65 degrees C), water (0-10 wt/wt%), and enzyme (5-20 wt/wt%) concentrations and oil-to-ethanol molar ratio (1:3 to 1:10). An empirical model was then built so as to assess the main and cross-variable effects on the reaction conversion and also to maximize biodiesel production for each enzyme. For the system containing Novozym 435 as catalyst the maximum conversion obtained was 81.4% at 65 degrees C, enzyme concentration of 20 wt/wt%, water concentration of 0 wt/wt%, and oil-to-ethanol molar ratio of 1:10. When the catalyst was Lipozyme IM, a conversion as high as 98% was obtained at 65 degrees C, enzyme concentration of 20 wt/wt%, water concentration of 0 wt/wt%, and oil-to-ethanol molar ratio of 1:3.
我们研究了以正己烷为溶剂，使用两种市售脂肪酶Novozym 435和Lipozyme IM作为催化剂，从蓖麻油生产脂肪酸乙酯的过程。为此，采用田口实验设计，考虑以下变量：温度（35 - 65摄氏度）、水（0 - 10 wt/wt%）、酶（5 - 20 wt/wt%）浓度以及油与乙醇的摩尔比（1:3至1:10）。然后建立了一个经验模型，以评估对反应转化率的主要和交叉变量影响，并使每种酶的生物柴油产量最大化。对于以Novozym 435为催化剂的体系，在65摄氏度、酶浓度为20 wt/wt%、水浓度为0 wt/wt%以及油与乙醇的摩尔比为1:10的条件下，获得的最大转化率为81.4%。当催化剂为Lipozyme IM时，在65摄氏度、酶浓度为20 wt/wt%、水浓度为0 wt/wt%以及油与乙醇的摩尔比为1:3的条件下，获得了高达98%的转化率。
