Skjeldal S, Grøgaard B, Nordsletten L, Reikerås O, Svindland A, Torvik A
Sophies Minde Orthopaedic Hospital, University of Oslo, Norway.
Eur Surg Res. 1992;24(4):197-203. doi: 10.1159/000129207.
In the present study, a rat hindlimb tourniquet model was used to investigate the effect of moderate hypothermia on ischemic muscle necrosis. Complete circulatory arrest was maintained for 4.5 h. During the ischemic period the animals were kept in an infant incubator at different temperatures. After 72 h survival the percentage of necrosis in the anterior tibial muscle was measured morphometrically on histological slides. At an ambient temperature of 24 degrees C there was 80% necrosis in the anterior tibial muscle. At 22 degrees C the necrosis was reduced to 29%. This reduction corresponds to more than 30 min shortening of the ischemia time. Differences in tissue temperature may explain some of the discrepancies reported in tolerance limits for muscle ischemia. To achieve consistent results in experimental muscle ischemia, it is necessary to control the ambient temperature.
在本研究中，使用大鼠后肢止血带模型来研究中度低温对缺血性肌肉坏死的影响。完全循环阻断维持4.5小时。在缺血期间，将动物置于不同温度的婴儿培养箱中。存活72小时后，在组织学切片上通过形态计量学测量胫前肌的坏死百分比。在环境温度为24摄氏度时，胫前肌坏死率为80%。在22摄氏度时，坏死率降至29%。这种降低相当于缺血时间缩短超过30分钟。组织温度的差异可能解释了在肌肉缺血耐受极限方面报道的一些差异。为了在实验性肌肉缺血中获得一致的结果，控制环境温度是必要的。
