Skjeldal S, Hvaal K, Nordsletten L, Aasen A O, Reikerås O, Torvik A
Institute for Surgical Research, Rikshospitalet, National Hospital, Oslo, Norway.
Eur Surg Res. 1994;26(2):94-100. doi: 10.1159/000129323.
Complete ischemia in the left hindlimb was maintained for 3.5 h in 16 rats randomized into two equal groups. One group served as control, the other was treated with pentoxifylline before, during and after the ischemic period. The animals were killed 72 h later, and the anterior tibial muscles were prepared for histological investigation. The most severely damaged muscles had a central core with complete necrosis of all muscle fibers, disintegrated capillaries and no macrophage infiltration. This zone, which was called the area of no resorption, was surrounded by an area of incomplete necrosis with partly resorbed muscle fibers, intact capillaries and marked macrophage infiltration. The muscles were completely intact only in a narrow subfascial zone. The total areas of necrosis and the areas of no resorption were measured as percent of the cross-sectional area of each muscle. There was extensive necrosis in both groups. The infarcted area was 93% in the control group and 89% in the treated group (NS). The corresponding areas of no resorption were 23 and 6% (p = 0.01). The study indicates that pentoxifylline has some protective effect on ischemic muscle damage. However, this effect is marginal compared to moderate hypothermic treatment.
将16只大鼠随机分为两组，每组8只，使左后肢完全缺血3.5小时。一组作为对照组，另一组在缺血期之前、期间和之后用己酮可可碱治疗。72小时后处死动物，取胫前肌准备进行组织学研究。受损最严重的肌肉有一个中央核心区，所有肌纤维完全坏死，毛细血管崩解，无巨噬细胞浸润。这个区域称为无吸收区，周围是不完全坏死区，肌纤维部分被吸收，毛细血管完整，有明显的巨噬细胞浸润。仅在狭窄的筋膜下区域肌肉完全完整。测量坏死总面积和无吸收区面积占每块肌肉横截面积的百分比。两组均有广泛坏死。对照组梗死面积为93%，治疗组为89%（无统计学差异）。相应的无吸收区面积分别为23%和6%（p = 0.01）。该研究表明己酮可可碱对缺血性肌肉损伤有一定的保护作用。然而，与中度低温治疗相比，这种作用很微弱。
