Yamaguchi Takako, Inoue Mayumi, Miyachi Kanako, Tominaga Hiroshi, Fujita Yoshikazu
Osaka University of Pharmaceutical Sciences, Nasahara, Takatsuki, Osaka 569-1094, Japan.
Anal Sci. 2004 Feb;20(2):387-9. doi: 10.2116/analsci.20.387.
A simple and highly sensitive spectrophotometric method for the determination of glucosamine and its analogous amino sugars was established based on fading of the palladium(II)-o-hydroxyhydroquinonephthalein-hexadecyltrimethylammonium complex. In the determination of glucosamine, Beer's law is obeyed in the range of 0.02 - 0.18 microg ml(-1), with an effective molar absorptivity at 630 nm and the relative standard deviation being 8.4 x 10(5) dm3 mol(-1) cm(-1) and 1.08% (n = 10). This method is about 70-times more sensitive than the Elson-Morgan method. The method was successfully applied to the assay of glucosamine in actual samples.
基于钯（II）-邻羟基氢醌酚酞-十六烷基三甲基铵配合物的褪色反应，建立了一种简单且高灵敏度的分光光度法，用于测定氨基葡萄糖及其类似的氨基糖。在氨基葡萄糖的测定中，在0.02 - 0.18微克/毫升范围内符合比尔定律，在630纳米处的有效摩尔吸光系数和相对标准偏差分别为8.4×10⁵立方分米/摩尔·厘米⁻¹和1.08%（n = 10）。该方法的灵敏度比埃尔森-摩根法高约70倍。该方法已成功应用于实际样品中氨基葡萄糖的测定。
