Hoshino Mitsuru, Yasui Hiroyuki, Sakurai Hiromu, Yamaguchi Takako, Fujita Yoshikazu
Osaka University of Pharmaceutical Sciences, Osaka, Japan.
Yakugaku Zasshi. 2011;131(7):1095-101. doi: 10.1248/yakushi.131.1095.
Simultaneous and fractional determination of iron(II) and iron(III) was accomplished with o-hydroxyhydroquinonephthalein (QP) in the presence of poly (N-vinyl pyrrolidone). In the determination of total iron (iron(II)+iron(III)), Beer's law was obeyed in the range of 0.02-0.67 μg·ml(-1), with an effective molar absorptivity (at 570 nm) and a relative standard deviation of 1.30×10(5)·l·mol(-1)·cm(-1) and 0.77% (n = 8), respectively. This method was about 10-15 times and more than the methods using 1,10-phenanthroline and 2,2'-bipyridine. In addition, the iron-QP complex was characterized using spectrophotometry and the electron spin resonance. This method was successfully applied to assays of total iron and iron(III) in pharmaceutical preparations.
在聚乙烯吡咯烷酮存在下，用邻羟基对苯二酚酚酞（QP）实现了铁（II）和铁（III）的同时及分步测定。在总铁（铁（II）+铁（III））的测定中，在0.02 - 0.67 μg·ml⁻¹范围内符合比尔定律，有效摩尔吸光系数（在570 nm处）和相对标准偏差分别为1.30×10⁵·l·mol⁻¹·cm⁻¹和0.77%（n = 8）。该方法比使用1,10 - 菲啰啉和2,2'-联吡啶的方法灵敏度高约10 - 15倍。此外，利用分光光度法和电子自旋共振对铁 - QP络合物进行了表征。该方法成功应用于药物制剂中总铁和铁（III）的测定。
