用邻羟基对苯二酚酚酞和锰（II）分光光度法测定精胺及相关化合物

Spectrophotometric determination of spermine and related compounds using o-hydroxyhydroquinonephthalein and manganese(II).

作者信息

Miyachi Kanako, Moriyama Kenzo, Yamaguchi Takako, Tominaga Hiroshi, Kamino Shinichiro, Fujita Yoshikazu

机构信息

Department of Hospital Pharmacy School of Medicine, Kinki University, Osaka, Japan.

出版信息

Anal Sci. 2007 Sep;23(9):1103-7. doi: 10.2116/analsci.23.1103.

DOI:10.2116/analsci.23.1103

PMID:17878586

Abstract

A simple and highly sensitive spectrophotometric method for the determination of spermine (Spm) was established based on the ternary complex formation reaction of Spm with o-hydroxyhydroquinonephthalein (QP) as a xanthene dye and manganese(II) as a metal ion in the presence of a dispersion agent. The apparent molar absorptivity at 555 nm and the relative standard deviation of the proposed method were 1.4 x 10(5) dm(3) mol(-1) cm(-1) and 0.50% (n = 10), respectively. In the method for flow-injection analysis (FIA), which employs a single-channel flow manifold system, a good linear relationship was observed over the 2 - 20 pg microl(-1) range of Spm by direct injection.

摘要

基于精胺（Spm）与作为呫吨染料的邻羟基对苯二酚酚酞（QP）以及金属离子锰（II）在分散剂存在下形成三元络合物的反应，建立了一种简单且高灵敏度的分光光度法用于测定精胺。该方法在555 nm处的表观摩尔吸光系数和相对标准偏差分别为1.4×10⁵ dm³ mol⁻¹ cm⁻¹和0.50%（n = 10）。在采用单通道流动池系统的流动注射分析（FIA）方法中，通过直接进样在2 - 20 pg μL⁻¹的精胺浓度范围内观察到了良好的线性关系。