Miyachi Kanako, Moriyama Kenzo, Yamaguchi Takako, Tominaga Hiroshi, Kamino Shinichiro, Fujita Yoshikazu
Department of Hospital Pharmacy School of Medicine, Kinki University, Osaka, Japan.
Anal Sci. 2007 Sep;23(9):1103-7. doi: 10.2116/analsci.23.1103.
A simple and highly sensitive spectrophotometric method for the determination of spermine (Spm) was established based on the ternary complex formation reaction of Spm with o-hydroxyhydroquinonephthalein (QP) as a xanthene dye and manganese(II) as a metal ion in the presence of a dispersion agent. The apparent molar absorptivity at 555 nm and the relative standard deviation of the proposed method were 1.4 x 10(5) dm(3) mol(-1) cm(-1) and 0.50% (n = 10), respectively. In the method for flow-injection analysis (FIA), which employs a single-channel flow manifold system, a good linear relationship was observed over the 2 - 20 pg microl(-1) range of Spm by direct injection.
基于精胺（Spm）与作为呫吨染料的邻羟基对苯二酚酚酞（QP）以及金属离子锰（II）在分散剂存在下形成三元络合物的反应，建立了一种简单且高灵敏度的分光光度法用于测定精胺。该方法在555 nm处的表观摩尔吸光系数和相对标准偏差分别为1.4×10⁵ dm³ mol⁻¹ cm⁻¹和0.50%（n = 10）。在采用单通道流动池系统的流动注射分析（FIA）方法中，通过直接进样在2 - 20 pg μL⁻¹的精胺浓度范围内观察到了良好的线性关系。
