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Computational and experimental identification of C. elegans microRNAs.秀丽隐杆线虫微小RNA的计算与实验鉴定
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优化RNA干扰技术以应用于哺乳动物细胞。

Optimizing RNA interference for application in mammalian cells.

作者信息

Medema René H

机构信息

Division of Molecular Biology, H8, Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands.

出版信息

Biochem J. 2004 Jun 15;380(Pt 3):593-603. doi: 10.1042/BJ20040260.

DOI:10.1042/BJ20040260
PMID:15056071
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1224236/
Abstract

Over the last 2 years, the scientific community has rapidly embraced novel technologies that allow gene silencing in vertebrates. Ease of application, cost effectiveness and the possibilities for genome-wide reverse genetics have quickly turned this approach into a widely accepted, almost mandatory asset for a self-respecting laboratory in life sciences. This review discusses some of the recent technological developments that allow the application of RNAi (RNA interference) in mammalian cells. In addition, the advantages of applying RNAi to study cell cycle events and the emerging approaches to perform mutational analysis by complementation in mammalian cells are evaluated. In addition, common pitfalls and drawbacks of RNAi will be reviewed, as well as the possible ways to get around these shortcomings of gene silencing by small interfering RNA.

摘要

在过去两年中，科学界迅速采用了能够在脊椎动物中实现基因沉默的新技术。这些技术应用简便、成本效益高，且具有全基因组反向遗传学的潜力，这使得该方法迅速成为生命科学领域有自尊心的实验室广泛接受且几乎不可或缺的工具。本综述讨论了一些最近的技术进展，这些进展使得RNA干扰（RNAi）能够应用于哺乳动物细胞。此外，还评估了应用RNAi研究细胞周期事件的优势以及在哺乳动物细胞中通过互补进行突变分析的新兴方法。此外，还将回顾RNAi常见的陷阱和缺点，以及克服小干扰RNA基因沉默这些缺点的可能方法。