Turunen Nina, Karihtala Peeter, Mantyniemi Anne, Sormunen Raija, Holmgren Arne, Kinnula Vuokko L, Soini Ylermi
Department of Pathology, Oulu University Hospital, Oulu, Finland.
APMIS. 2004 Feb;112(2):123-32. doi: 10.1111/j.1600-0463.2004.apm1120207.x.
We investigated the expression of thioredoxin and thioredoxin reductase in a large set of breast invasive and in situ carcinomas by immunohistochemistry. Additionally, NF-kappa B, p53 and proliferating cell nuclear antigen (PCNA) expression was studied. Thioredoxin and thioredoxin reductase expression was located in both cytoplasmic and nuclear compartments of the cell. Cytoplasmic thioredoxin positivity was found in 67 % and nuclear in 59 % of the cases, while thioredoxin reductase was found in 55 % and 6 % of cases, respectively. Ductal carcinomas showed stronger cytoplasmic thioredoxin immunoreactivity than lobular ones. Nuclear thioredoxin positivity was more often found in in situ lesions, and lobular carcinomas were more often negative than ductal ones. Both cytoplasmic and nuclear thioredoxin-positive cases had a high proliferation measured by PCNA staining. Positive nuclear immunostaining was associated with negative estrogen and progesterone receptor status. Cases with high p53 expression showed significantly higher nuclear thioredoxin positivity, but lower thioredoxin reductase positivity. Whilst thioredoxin or thioredoxin reductase was not associated with patient survival, cases showing both cytoplasmic and nuclear thioredoxin reductase-positive tumours had a shorter disease-free interval than those with negative immunostaining.
我们通过免疫组织化学方法研究了大量乳腺浸润性癌和原位癌中硫氧还蛋白和硫氧还蛋白还原酶的表达情况。此外，还研究了核因子κB、p53和增殖细胞核抗原（PCNA）的表达。硫氧还蛋白和硫氧还蛋白还原酶的表达位于细胞的细胞质和细胞核区室。在67%的病例中发现细胞质硫氧还蛋白呈阳性，59%的病例中细胞核呈阳性，而硫氧还蛋白还原酶分别在55%和6%的病例中被发现。导管癌的细胞质硫氧还蛋白免疫反应性比小叶癌更强。细胞核硫氧还蛋白阳性更常见于原位病变，小叶癌比导管癌更常呈阴性。通过PCNA染色测量，细胞质和细胞核硫氧还蛋白阳性的病例均具有较高的增殖率。细胞核免疫染色阳性与雌激素和孕激素受体状态阴性相关。p53高表达的病例显示细胞核硫氧还蛋白阳性显著更高，但硫氧还蛋白还原酶阳性更低。虽然硫氧还蛋白或硫氧还蛋白还原酶与患者生存率无关，但细胞质和细胞核硫氧还蛋白还原酶均呈阳性的肿瘤病例的无病生存期比免疫染色阴性的病例更短。
