Yokoi F, Sugiura M
Center for Gene Research, Nagoya University, Japan.
FEBS Lett. 1992 Aug 24;308(3):258-60. doi: 10.1016/0014-5793(92)81287-v.
The genes for ribosomal proteins L28 and L33 constitute an operon (rpmBG) in E. coli, but in plant chloroplasts L33 is encoded by the chloroplast DNA and L28 seems to be encoded by the nuclear genome. A 15 kDa protein was isolated from the 50 S subunit of tobacco chloroplast ribosomes and its N-terminal amino acid sequence was determined. A cDNA for this protein was cloned and analyzed. The cDNA encodes a 151 amino acid protein consisting of a predicted transit-peptide of 74 amino acids and a mature protein of 77 amino acids. The mature protein is homologous to E. coli L28, hence we named it chloroplast L28 (CL28). This is the first report on the presence of an E. coli L28-like protein in another organism.
核糖体蛋白L28和L33的基因在大肠杆菌中构成一个操纵子（rpmBG），但在植物叶绿体中，L33由叶绿体DNA编码，而L28似乎由核基因组编码。从烟草叶绿体核糖体的50 S亚基中分离出一种15 kDa的蛋白质，并测定了其N端氨基酸序列。克隆并分析了该蛋白质的cDNA。该cDNA编码一个由74个氨基酸的预测转运肽和77个氨基酸的成熟蛋白组成的151个氨基酸的蛋白质。成熟蛋白与大肠杆菌L28同源，因此我们将其命名为叶绿体L28（CL28）。这是关于另一种生物体中存在大肠杆菌L28样蛋白的首次报道。
