叶绿体核糖体蛋白L32由叶绿体基因组编码。
Chloroplast ribosomal protein L32 is encoded in the chloroplast genome.
作者信息
Yokoi F, Vassileva A, Hayashida N, Torazawa K, Wakasugi T, Sugiura M
机构信息
Center for Gene Research, Nagoya University, Japan.
出版信息
FEBS Lett. 1990 Dec 10;276(1-2):88-90. doi: 10.1016/0014-5793(90)80514-j.
The 50 S subunit of chloroplast ribosomes was prepared from tobacco leaves. The proteins were fractionated and the N-terminal amino acid sequence of a 14 kDa protein was determined. This sequence matches the N-terminal sequence deduced from ORF55 located between ndhF and trnL on the small single-copy region of tobacco chloroplast DNA. The deduced protein shows homology to E. coli and B. stearothermophilus L32 proteins, and it has been named as CL32 and ORF55 as rpl32. The tobacco chloroplast genome therefore contains 21 different ribosomal protein genes.
叶绿体核糖体的50 S亚基是从烟草叶片中制备的。对蛋白质进行了分级分离，并测定了一种14 kDa蛋白质的N端氨基酸序列。该序列与从烟草叶绿体DNA小单拷贝区域中ndhF和trnL之间的ORF55推导的N端序列相匹配。推导的蛋白质与大肠杆菌和嗜热栖热菌的L32蛋白质具有同源性，它被命名为CL32，ORF55被命名为rpl32。因此，烟草叶绿体基因组包含21个不同的核糖体蛋白基因。
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