Tanaka M, Wakasugi T, Sugita M, Shinozaki K, Sugiura M
Proc Natl Acad Sci U S A. 1986 Aug;83(16):6030-4. doi: 10.1073/pnas.83.16.6030.
The nucleotide sequence of a tobacco (Nicotiana tabacum) chloroplast gene cluster that encodes eight proteins homologous to Escherichia coli ribosomal proteins L23, L2, S19, L22, S3, L16, L14, and S8 has been determined. RNA gel blot hybridization revealed that all eight coding regions are expressed in the chloroplasts. The arrangement of the eight genes resembles that found in the E. coli S10 and spc operons. Among the eight genes, the L2 and L16 genes contain 666- and 1020-base-pair introns, respectively. These intron boundary sequences are consistent with the conserved boundary sequences of the chloroplast group III introns [Shinozaki, K., Deno, H., Sugita, M., Kuramitsu, S. & Sugiura, M. (1986) Mol. Gen. Genet. 202, 1-5].
已确定一个烟草(烟草属)叶绿体基因簇的核苷酸序列,该基因簇编码八种与大肠杆菌核糖体蛋白L23、L2、S19、L22、S3、L16、L14和S8同源的蛋白质。RNA凝胶印迹杂交显示,所有八个编码区均在叶绿体中表达。这八个基因的排列方式类似于在大肠杆菌S10和spc操纵子中发现的排列方式。在这八个基因中,L2和L16基因分别含有666个和1020个碱基对的内含子。这些内含子边界序列与叶绿体III类内含子的保守边界序列一致[Shinozaki, K., Deno, H., Sugita, M., Kuramitsu, S. & Sugiura, M. (1986) Mol. Gen. Genet. 202, 1 - 5]。