Tavechio Ana Terezinha, Marques Lilian Regina M, Abe Cecília Mari, Gomes Tânia A T
Seção de Bacteriologia, Instituto Adolfo Lutz, São Paulo, SP, 01246-902, Brasil.
Mem Inst Oswaldo Cruz. 2004 Feb;99(1):81-3. doi: 10.1590/s0074-02762004000100014. Epub 2004 Mar 31.
The enteropathogenic role of cytotoxic necrotizing factor (CNF)-producing Escherichia coli was investigated by searching cnf genes among 2074 isolates from 200 children with and 200 without acute diarrhea in Brazil. Fourteen (7%) cases versus 10 (5%) control children carried at least one cnf positive isolate (P = 0.50) and most isolates expressed CNF type 1. DNA sequences of virulence factors of extraintestinal pathogenic E. coli (ExPEC) were detected in 78.6% of CNF1-producing isolates. Besides not being associated with human acute diarrhea, the CNF1-producing isolates here identified may represent potential ExPEC transitorily composing the normal intestinal flora.
通过在巴西200名患急性腹泻和200名未患急性腹泻儿童的2074株分离菌株中搜索cnf基因，研究了产细胞毒性坏死因子（CNF）的大肠杆菌的肠道致病作用。14名（7%）病例与10名（5%）对照儿童携带至少一株cnf阳性分离菌株（P = 0.50），且大多数分离菌株表达1型CNF。在78.6%的产CNF1分离菌株中检测到肠外致病性大肠杆菌（ExPEC）毒力因子的DNA序列。这里鉴定出的产CNF1分离菌株除了与人类急性腹泻无关外，可能代表暂时构成正常肠道菌群的潜在ExPEC。
