针对金黄色葡萄球菌ATCC 12600过氧化氢酶的多克隆抗体无法识别金黄色葡萄球菌厌氧亚种细胞提取物中的任何蛋白质。

Polyclonal antibodies against Staphylococcus aureus ATCC 12600 catalase do not recognize any protein in cellular extracts from S. aureus subsp. anaerobius.

作者信息

Ruiz Santa Quiteria J A, Cid D, Bellahsene R, Suárez G, de la Fuente R

机构信息

Departamento Patología Animal I, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.

出版信息

FEMS Microbiol Lett. 1992 Jun 1;72(2):173-6. doi: 10.1016/0378-1097(92)90524-r.

DOI:10.1016/0378-1097(92)90524-r

PMID:1505741

Abstract

Rabbits were immunized with electrophoretically pure catalase from Staphylococcus aureus ATCC 12 600. The antiserum was used to study whether S. aureus subsp. anaerobius was able to synthesize the apoprotein of catalase. Proteins were separated on polyacrylamide gels (SDS-PAGE) and transferred to nitrocellulose membranes and were detected by immunoblotting. No protein reacting with the purified immunoglobulins against S. aureus ATCC 12,600 catalase could be detected in crude and partially purified cellular extracts from S. aureus subsp. anaerobius or its aerotolerant mutants.

摘要

用来自金黄色葡萄球菌ATCC 12600的电泳纯过氧化氢酶对兔子进行免疫。该抗血清用于研究金黄色葡萄球菌厌氧亚种是否能够合成过氧化氢酶的脱辅基蛋白。蛋白质在聚丙烯酰胺凝胶（SDS-PAGE）上分离，转移到硝酸纤维素膜上，并通过免疫印迹法进行检测。在金黄色葡萄球菌厌氧亚种或其耐氧突变体的粗提物和部分纯化的细胞提取物中，未检测到与针对金黄色葡萄球菌ATCC 12600过氧化氢酶的纯化免疫球蛋白发生反应的蛋白质。