Elbir Haitham, Robert Catherine, Nguyen Ti Thien, Gimenez Grégory, El Sanousi Sulieman M, Flock Jan-Ingmar, Raoult Didier, Drancourt Michel
Aix Marseille Université, URMITE, Marseille, France.
Faculty of Veterinary Medicine, University of Khartoum, Sudan.
Stand Genomic Sci. 2013 Oct 15;9(1):1-13. doi: 10.4056/sigs.3748294. eCollection 2013 Oct 16.
Staphylococcus aureus subsp. anaerobius is responsible for Morel's disease in animals and a cause of abscess in humans. It is characterized by a microaerophilic growth, contrary to the other strains of S. aureus. The 2,604,446-bp genome (32.7% GC content) of S. anaerobius ST1464 comprises one chromosome and no plasmids. The chromosome contains 2,660 open reading frames (ORFs), 49 tRNAs and three complete rRNAs, forming one complete operon. The size of ORFs ranges between 100 to 4,600 bp except for two ORFs of 6,417 and 7,173 bp encoding segregation ATPase and non-ribosomal peptide synthase, respectively. The chromosome harbors Staphylococcus phage 2638A genome and incomplete Staphylococcus phage genome PT1028, but no detectable CRISPRS. The antibiotic resistance gene for tetracycline was found although Staphylococcus aureus subsp. anaerobius is susceptible to tetracycline in-vitro. Intact oxygen detoxification genes encode superoxide dismutase and cytochrome quinol oxidase whereas the catalase gene is impaired by a stop codon. Based on the genome, in-silico multilocus sequence typing indicates that S. aureus subsp. anaerobius emerged as a clone separated from all other S. aureus strains, illustrating host-adaptation linked to missing functions. Availability of S. aureus subsp. anaerobius genome could prompt the development of post-genomic tools for its rapid discrimination from S. aureus.
金黄色葡萄球菌厌氧亚种可引发动物的莫雷尔氏病以及人类的脓肿。与其他金黄色葡萄球菌菌株不同，其特点是微需氧生长。金黄色葡萄球菌厌氧亚种ST1464的2,604,446碱基对基因组（GC含量为32.7%）由一条染色体组成，无质粒。该染色体包含2,660个开放阅读框（ORF）、49个tRNA和三个完整的rRNA，形成一个完整的操纵子。除了分别编码分离ATP酶和非核糖体肽合成酶的两个6,417和7,173碱基对的ORF外，ORF的大小在100至4,600碱基对之间。该染色体含有葡萄球菌噬菌体2638A基因组和不完整的葡萄球菌噬菌体基因组PT1028，但未检测到CRISPRS。尽管金黄色葡萄球菌厌氧亚种在体外对四环素敏感，但仍发现了四环素抗性基因。完整的氧解毒基因编码超氧化物歧化酶和细胞色素喹啉氧化酶，而过氧化氢酶基因因一个终止密码子而受损。基于基因组，计算机多位点序列分型表明，金黄色葡萄球菌厌氧亚种是从所有其他金黄色葡萄球菌菌株中分离出来的一个克隆，说明了与缺失功能相关的宿主适应性。金黄色葡萄球菌厌氧亚种基因组的可用性可能会促使开发用于快速区分它与金黄色葡萄球菌的后基因组工具。
