Royds J A, Sharrard R M, Parsons M A, Lawry J, Rees R, Cottam D, Wagner B, Rennie I G
Department of Pathology, Sheffield University Medical School, United Kingdom.
Graefes Arch Clin Exp Ophthalmol. 1992;230(4):366-71. doi: 10.1007/BF00165947.
We have investigated the expression of c-myc in 24 ocular melanomas by immunohistochemistry, using two monoclonal antibodies raised against a mid-sequence portion of the c-myc product (6E10) and against the C-terminus (9E10). The results were compared with other putative prognostic factors, including tumour size, cell type, proliferation index (determined by flow cytometry), and ploidy, as well as immunohistochemical staining for HMB-45 and S-100 antigens. Staining, often focal, for c-myc was found in both the nucleus and the cytoplasm of a proportion of the cells in most tumours studied. Total cell staining for myc protein correlated with proliferative index in diploid tumours; seven out of nine aneuploid and mixed aneuploid/diploid cells showed strong staining in at least one cellular compartment. A positive correlation with myc expression was also found for HMB-45 staining, but not for cell type or staining for S-100. The results support the hypothesis that myc protein is involved in cellular proliferation in uveal melanomas and indicate that immunohistochemistry for myc antigen may be a useful prognostic marker in these tumours.
我们运用免疫组织化学方法，使用两种分别针对c-myc产物中间序列部分（6E10）和C末端（9E10）制备的单克隆抗体，对24例眼黑色素瘤中c-myc的表达情况进行了研究。将结果与其他假定的预后因素进行了比较，这些因素包括肿瘤大小、细胞类型、增殖指数（通过流式细胞术测定）、倍性，以及HMB-45和S-100抗原的免疫组织化学染色。在大多数所研究的肿瘤中，部分细胞的细胞核和细胞质中均发现了c-myc染色，且通常呈局灶性。在二倍体肿瘤中，myc蛋白的总细胞染色与增殖指数相关；9例非整倍体及非整倍体/二倍体混合细胞中有7例在至少一个细胞区室中显示强染色。HMB-45染色也与myc表达呈正相关，但与细胞类型或S-100染色无关。这些结果支持了myc蛋白参与葡萄膜黑色素瘤细胞增殖的假说，并表明myc抗原的免疫组织化学检测可能是这些肿瘤中一种有用的预后标志物。
