Stavropoulos Andreas, Kostopoulos Lambros, Nyengaard Jens Randel, Karring Thorkild
Department of Periodontology and Oral Gerontology, Faculty of Health Sciences, Royal Dental College, University of Aarhus, Denmark.
J Clin Periodontol. 2004 Jan;31(1):30-9. doi: 10.1111/j.0303-6979.2004.00434.x.
The aim of the study was to examine whether bone produced by guided tissue regeneration (GTR) in combination with Bio-Oss or Biogran is stable on a long-term basis.
Fifty-four, 3-month-old Wistar rats were divided into three groups and rigid, hemispherical, teflon capsules were placed with their open part facing the lateral surface of the exposed mandibular ramus (one capsule per animal). In the first group, the capsules were loosely packed with a standardized quantity of Bio-Oss, in the second group with Biogran, and in the last group were left empty. After 1 year of healing, the capsules were removed. Six animals from each of the 3 experimental groups were killed immediately after capsule removal (baseline), or 3 or 6 months after re-entry. The volume of (1) newly formed bone, (2) remaining biomaterial particles, and (3) soft connective tissue in the space originally created by the capsule was estimated by a point-counting technique in three to four histological sections, taken by uniformly random sampling.
While considerable bone formation had occurred in the empty control capsules, only limited bone formation was observed in the two test groups. The major portion of the space originally created by the capsules in the test groups was occupied by biomaterial particles embedded in connective tissue. At baseline, the mean volume of newly formed bone occupied 23% of the original capsule space in the animals grafted with Bio-Oss, 12.6% in those implanted with Biogran, and 94.1% in those that received empty control capsules. Six months after capsule removal, the corresponding values were 21.5%, 13.2%, and 91.7%, respectively. No statistically significant differences were observed between baseline, and the 3-, and 6-month observation times in terms of bone volume for any of the three treatment groups (p>0.05).
Bone produced by GTR with and without implantation of Bio-Oss, or Biogran, is stable on a long-term basis, but bone formation is obstructed by implantation of these biomaterials.
本研究旨在探讨引导组织再生（GTR）联合Bio-Oss或Biogran所形成的骨在长期内是否稳定。
将54只3月龄的Wistar大鼠分为三组，将刚性、半球形的聚四氟乙烯胶囊开口部分朝向暴露的下颌支外侧表面放置（每只动物放置一个胶囊）。第一组，胶囊内松散填充标准量的Bio-Oss；第二组填充Biogran；最后一组为空胶囊。愈合1年后，取出胶囊。3个实验组每组各6只动物在取出胶囊后立即（基线）处死，或在再次切开后3个月或6个月处死。通过在三到四个组织学切片上采用点计数技术，对（1）新形成的骨、（2）剩余的生物材料颗粒以及（3）胶囊最初所占据空间内的软结缔组织体积进行估计，切片采用均匀随机抽样获取。
虽然空的对照胶囊内发生了大量骨形成，但在两个试验组中仅观察到有限的骨形成。试验组中胶囊最初所占据空间的大部分被嵌入结缔组织中的生物材料颗粒占据。在基线时，植入Bio-Oss的动物中新形成骨的平均体积占原始胶囊空间的23%，植入Biogran的动物中占12.6%，接受空对照胶囊的动物中占94.1%。取出胶囊6个月后，相应的值分别为21.5%、13.2%和91.7%。对于三个治疗组中的任何一组，在基线以及3个月和6个月观察期的骨体积方面均未观察到统计学上的显著差异（p>0.05）。
无论是否植入Bio-Oss或Biogran，GTR所形成的骨在长期内是稳定的，但这些生物材料的植入阻碍了骨形成。
