Department of Diagnosis and Surgery, School of Dentistry of Araraquara, Univ. Est. Paulista, Araraquara, Brazil.
Department of Oral Pathology, School of Dentistry of Araraquara, Univ. Est. Paulista, Araraquara, Brazil.
Clin Oral Investig. 2019 Apr;23(4):1605-1614. doi: 10.1007/s00784-018-2588-6. Epub 2018 Aug 25.
To evaluate the effect of grafting with strontium (Sr)-loaded deproteinized bovine bone (DBB) on bone healing in calvarial critical size defects (CSD) in rats.
Two circular bone defects (5 mm in diameter) were created in the calvaria of 42 rats. One of the defects, randomly chosen, was grafted with (a) DBB, (b) DBB loaded with 19.6 μg/g of Sr (DBB/Sr1), or (c) DBB loaded with 98.1 μg/g of Sr (DBB/Sr2). The other defect was left empty as negative control. Groups of seven animals from each of the groups were euthanized 15 and 60 days post-op. Bone healing in the CSD was evaluated by micro-CT and histology/histomorphometry and immunohistochemistry.
DBB/Sr2-grafted sites showed statistically significantly shorter radiographic residual defect length compared with DBB/Sr1- and DBB-grafted sites, and with empty controls at 60 days. Further, the amount of new bone formation in the DBB/Sr1- and DBB/Sr2-grafted sites was significantly higher compared with that in the DBB-grafted sites at 60 days. A larger number of DBB/Sr1- and DBB/Sr2-grafted sites presented with no- or only limited to mild inflammation, compared with the DBB-grafted sites, especially at 60 days. Higher expression of osteocalcin was observed in DBB/Sr1- and DBB/Sr2-grafted sites as compared to DBB-grafted sites.
Grafting with Sr-loaded DBB enhanced bone formation in CSD in rats, when compared with grafting with non-loaded DBB.
Grafting with Sr-loaded DBB may enhance bone formation in bone defects.
评估负载锶（Sr）去蛋白牛骨（DBB）移植物对大鼠颅骨临界尺寸缺损（CSD）骨愈合的影响。
在 42 只大鼠颅骨上制作两个直径为 5mm 的圆形骨缺损。随机选择其中一个缺损，分别用（a）DBB、（b）负载 19.6μg/g Sr 的 DBB（DBB/Sr1）或（c）负载 98.1μg/g Sr 的 DBB（DBB/Sr2）进行移植。另一个缺损作为阴性对照不做处理。每组的 7 只动物在术后 15 天和 60 天被处死。通过 micro-CT 和组织学/组织形态计量学及免疫组织化学评估 CSD 中的骨愈合情况。
与 DBB/Sr1 和 DBB 移植组以及空载体对照组相比，DBB/Sr2 移植组在术后 60 天时的放射学残余缺损长度明显更短。进一步，在术后 60 天时，DBB/Sr1 和 DBB/Sr2 移植组的新生骨形成量明显高于 DBB 移植组。与 DBB 移植组相比，DBB/Sr1 和 DBB/Sr2 移植组有更多的缺损部位表现为无或仅有轻微炎症，尤其是在术后 60 天。与 DBB 移植组相比，DBB/Sr1 和 DBB/Sr2 移植组的骨钙素表达更高。
与非负载 DBB 移植相比，负载 Sr 的 DBB 移植可增强大鼠 CSD 中的骨形成。
负载 Sr 的 DBB 移植可能会增强骨缺损中的骨形成。
