Franĕk J, Malina J, Krátká H
Institute of Experimental Medicine, Czechoslovak Academy of Sciences, Prague.
Folia Microbiol (Praha). 1992;37(2):146-52. doi: 10.1007/BF02836620.
Interactions between bacteria and the host were studied from day 0 up to day 10 post-challenge in mice pretreated with soluble glucan (20 mg/kg i.p.) and challenged supralaryngeally with a virulent strain of Klebsiella pneumoniae. In the initial phase of infection, clearance of bacteria in the airways of glucan-treated mice was improved to an extent comparable with the vaccinated group but, in contrast to the immunized animals, subsequent regrowth of the bacterial inoculum was not prevented. The efficacy of defense, based during the entire course of infection mainly upon phagocytosis by neutrophils, markedly increased at intervals corresponding to the onset of humoral immune response. No evidence was obtained to indicate an enhanced involvement of alveolar macrophages in the phagocytosis of bacteria in glucan-stimulated mice. The results further support the notion that improvement of specific immune responsiveness rather than activation of nonspecific effector functions might be the most important expression of the host-defense-potentiating capacity of glucan and related stimulants of microbial origin.
在预先经可溶性葡聚糖（20毫克/千克腹腔注射）处理并用肺炎克雷伯菌强毒株进行喉上接种攻击的小鼠中，研究了从攻击后第0天到第10天细菌与宿主之间的相互作用。在感染的初始阶段，葡聚糖处理小鼠气道内细菌的清除得到改善，程度与接种疫苗组相当，但与免疫动物不同的是，随后接种细菌的再生长并未得到阻止。在感染的整个过程中，防御功效主要基于中性粒细胞的吞噬作用，在与体液免疫反应开始相对应的时间间隔显著增加。没有证据表明在葡聚糖刺激的小鼠中肺泡巨噬细胞参与细菌吞噬的情况有所增强。结果进一步支持了这样一种观点，即特异性免疫反应性的改善而非非特异性效应功能的激活可能是葡聚糖及相关微生物来源刺激物增强宿主防御能力的最重要表现。
