Libich J, Franĕk J, Hásková V
Folia Microbiol (Praha). 1983;28(5):424-9. doi: 10.1007/BF02879494.
An experimental model in white mice, infected with a mildly virulent strain of Klebsiella pneumoniae, was elaborated for studies on local immunity of the respiratory tract. Instillation of klebsiella into the supralaryngeal space of anaesthetised animals proved to be more suitable than the commonly used method of intranasal infection. The strain administered by the supralaryngeal route, persisted in the lungs of most mice at approximately equal level 1 d after infection, in some animals it could be demonstrated even after 2-3 d. Using this model (based on various rates of lung clearance), one can demonstrate faster elimination of klebsiella after a local (supralaryngeal) than systemic (intraperitoneal) immunization with a heat-inactivated vaccine, prepared from a homologous strain of K. pneumoniae.
建立了一个用轻度毒力的肺炎克雷伯菌感染的小白鼠实验模型，用于研究呼吸道局部免疫。将克雷伯菌滴入麻醉动物的喉上间隙，结果证明比常用的鼻内感染方法更合适。通过喉上途径给药的菌株，在感染后1天，大多数小鼠的肺部以大致相同的水平持续存在，在一些动物中，甚至在2 - 3天后仍可检测到。使用这个模型（基于不同的肺部清除率），可以证明，用由肺炎克雷伯菌同源菌株制备的热灭活疫苗进行局部（喉上）免疫比全身（腹腔内）免疫后，克雷伯菌的清除速度更快。
