Guo Zheng, Sun Yan
Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
Biotechnol Prog. 2004 Mar-Apr;20(2):619-22. doi: 10.1021/bp034212m.
An operation mode with N(2) bubbling under vacuum was employed for the solvent-free synthesis of 1,3-diconjugated linoleoyl glycerol (1,3-dCLG) from conjugated linoleic acid (CLA) catalyzed by Novozym 435. The response surface methodology (RSM) was adopted for the optimization of the reaction conditions with five major factors (incubation time, temperature, enzyme load, substrate mole ratio, and system vacuum) and three responses (CLA conversion, 1,3-dCLG yield, and acyl migration). Two sets of optimal conditions were recommended. Validation of the RSM model was verified by the good agreement between the experimental and the predicted values of 1,3-dCLG yield. Under the optimal conditions, the yield of 1,3-dCLG up to 93% was obtained. The reaction was scaled up to a production level of 100 g of 1,3-dCLG at a yield of 90.7%, indicating a promising feature of the technology in industrial applications.
采用在真空下N(2)鼓泡的操作模式，以诺维信435为催化剂，从共轭亚油酸（CLA）出发进行无溶剂合成1,3 - 二共轭亚油酰甘油（1,3 - dCLG）。采用响应面法（RSM）对反应条件进行优化，考察了五个主要因素（孵育时间、温度、酶负载量、底物摩尔比和系统真空度）和三个响应指标（CLA转化率、1,3 - dCLG产率和酰基迁移）。推荐了两组最佳条件。通过1,3 - dCLG产率的实验值与预测值之间的良好一致性验证了RSM模型。在最佳条件下，1,3 - dCLG的产率高达93%。该反应放大至100 g 1,3 - dCLG的生产规模，产率为90.7%，表明该技术在工业应用中具有良好的前景。
