Dasuri Kumar, Antonovici Mihaela, Chen Keding, Wong Ken, Standing Kenneth, Ens Werner, El-Gabalawy Hani, Wilkins John A
Rheumatic Diseases Research Laboratory, University of Manitoba, Winnipeg, Canada.
Arthritis Res Ther. 2004;6(2):R161-8. doi: 10.1186/ar1153. Epub 2004 Feb 16.
The present studies were initiated to determine the protein expression patterns of fibroblast-like synovial (FLS) cells derived from the synovia of rheumatoid arthritis patients. The cellular proteins were separated by two-dimensional polyacrylamide gel electrophoresis and the in-gel digested proteins were analyzed by matrix-assisted laser desorption ionization mass spectrometry. A total of 368 spots were examined and 254 identifications were made. The studies identified a number of proteins that have been implicated in the normal or pathological FLS function (e.g. uridine diphosphoglucose dehydrogenase, galectin 1 and galectin 3) or that have been characterized as potential autoantigens in rheumatoid arthritis (e.g. BiP, colligin, HC gp-39). A novel uncharacterized protein product of chromosome 19 open reading frame 10 was also detected as an apparently major component of FLS cells. These results demonstrate the utility of high-content proteomic approaches in the analysis of FLS composition.
开展本研究以确定源自类风湿性关节炎患者滑膜的成纤维样滑膜（FLS）细胞的蛋白质表达模式。通过二维聚丙烯酰胺凝胶电泳分离细胞蛋白质，并用基质辅助激光解吸电离质谱分析凝胶内消化的蛋白质。共检测了368个斑点，进行了254次鉴定。这些研究鉴定出了一些与FLS正常或病理功能相关的蛋白质（如尿苷二磷酸葡萄糖脱氢酶、半乳糖凝集素1和半乳糖凝集素3），或已被表征为类风湿性关节炎潜在自身抗原的蛋白质（如结合蛋白、胶原结合蛋白、HC gp-39）。还检测到19号染色体开放阅读框10的一种新的未表征蛋白质产物，它显然是FLS细胞的主要成分。这些结果证明了高含量蛋白质组学方法在分析FLS组成中的实用性。
