Sinz Andrea, Bantscheff Marcus, Mikkat Stefan, Ringel Bruno, Drynda Susanne, Kekow Jörn, Thiesen Hans-Jürgen, Glocker Michael O
Proteome Center Rostock, Medical Faculty, University of Rostock, Germany.
Electrophoresis. 2002 Sep;23(19):3445-56. doi: 10.1002/1522-2683(200210)23:19<3445::AID-ELPS3445>3.0.CO;2-J.
Differential proteome analysis is used to study body fluids from patients suffering from rheumatoid arthritis (RA), reactive arthritis (reaA) or osteoarthritis (OA). Mass spectrometric structure characterization of gel-separated proteins provided a detailed view of the protein-processing events that lead to distinct protein species present in the respective body fluids. (i) Fibrin(ogen) beta-chain degradation products, presumably plasmin-derived, appeared solely in synovial fluids (SF) from both patient collectives, (ii) calgranulin B (MRP14) was exclusively identified in SF samples derived from 5 out of 6 patients suffering from RA. Calgranulin B was not observed in synovial fluids from OA patients, nor in plasmas from either patient group. In all cases where calgranulin B was detected, calgranulin C was identified as well. (iii) Serum amyloid A protein spots were determined in plasmas and synovial fluids from patients with RA, but not in patients with OA. In addition to disease-relevant differences, interindividual differences in haptoglobin patterns of the patients under investigation were observed. Hence, in-depth proteome analysis of body fluids has proven effective for identification of multiple molecular markers and determination of associated protein structure modifications, that are thought to play a role for specifically determining a defined pathological state of diseased joints.
差异蛋白质组分析用于研究类风湿性关节炎（RA）、反应性关节炎（reaA）或骨关节炎（OA）患者的体液。凝胶分离蛋白质的质谱结构表征提供了导致各自体液中存在不同蛋白质种类的蛋白质加工事件的详细视图。（i）纤维蛋白（原）β链降解产物，推测为纤溶酶衍生，仅出现在两个患者群体的滑液（SF）中；（ii）钙粒蛋白B（MRP14）仅在6例RA患者中的5例的SF样本中被鉴定出。在OA患者的滑液中未观察到钙粒蛋白B，在两个患者组的血浆中也未观察到。在所有检测到钙粒蛋白B的病例中，也鉴定出了钙粒蛋白C。（iii）在RA患者的血浆和滑液中测定了血清淀粉样蛋白A蛋白斑点，但在OA患者中未测定。除了与疾病相关的差异外，还观察到了所研究患者个体间触珠蛋白模式的差异。因此，体液的深度蛋白质组分析已被证明可有效鉴定多种分子标志物并确定相关的蛋白质结构修饰，这些修饰被认为在明确患病关节的特定病理状态中起作用。
