早期滑膜关节发育的单细胞转录组图谱。
A single cell transcriptional atlas of early synovial joint development.
机构信息
Institute for Cell Engineering, Johns Hopkins School of Medicine, Baltimore MD 21205, USA.
Department of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore MD 21205, USA.
出版信息
Development. 2020 Jul 20;147(14):dev185777. doi: 10.1242/dev.185777.
Synovial joint development begins with the formation of the interzone, a region of condensed mesenchymal cells at the site of the prospective joint. Recently, lineage-tracing strategies have revealed that Gdf5-lineage cells native to and from outside the interzone contribute to most, if not all, of the major joint components. However, there is limited knowledge of the specific transcriptional and signaling programs that regulate interzone formation and fate diversification of synovial joint constituents. To address this, we have performed single cell RNA-Seq analysis of 7329 synovial joint progenitor cells from the developing murine knee joint from E12.5 to E15.5. By using a combination of computational analytics, hybridization and characterization of prospectively isolated populations, we have identified the transcriptional profiles of the major developmental paths for joint progenitors. Our freely available single cell transcriptional atlas will serve as a resource for the community to uncover transcriptional programs and cell interactions that regulate synovial joint development.
滑液关节的发育始于间充质细胞带（interzone）的形成，该区域是未来关节部位的细胞密集区。最近，谱系追踪策略揭示了源自间充质细胞带内外的 Gdf5 谱系细胞有助于形成大多数（如果不是全部的话）主要关节成分。然而，对于调控间充质细胞带形成和滑膜关节成分命运多样化的特定转录和信号程序知之甚少。为了解决这个问题，我们对 E12.5 至 E15.5 发育中的小鼠膝关节中的 7329 个滑膜关节祖细胞进行了单细胞 RNA-Seq 分析。通过结合计算分析、杂交和预期分离群体的特征，我们确定了关节祖细胞的主要发育途径的转录特征。我们提供的免费单细胞转录图谱将作为一个资源，供社区用于揭示调控滑膜关节发育的转录程序和细胞相互作用。
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