生物传感器技术用于检测内毒素中和材料的研究
[Study of biosensor technology on the detection of endotoxin-neutralizing materials].
作者信息
Lu Gen-fa, Gong Xiao-yun, Wei Guo, Wang Ning, Xiao Guang-xia, Zheng Jiang
机构信息
Institute of Burn Research, Southwestern Hospital, State Key Laboratory of Trauma, Burns and Combined Injury, The Third Military Medical University, Chongqing 400038, P.R. China.
出版信息
Zhonghua Shao Shang Za Zhi. 2004 Feb;20(1):23-5.
OBJECTIVE
To explore the application of biosensor technology in the determination of endotoxin-neutralizing materials.
METHODS
After mixing polymyxin B (PMB) with endotoxin in certain concentration, the neutralizing ratio of PMB to endotoxin was assessed by biosensor technique and limulus amebocyte lysate test respectively. The results from the two methods were compared.
RESULTS
The neutralizing ratio of PMB to endotoxin as assessed by biosensor technology was 0.35 microg to 1 ng, while that by dynamic turbidimetric and chromogenic limulus amebocyte lysate (LAL) technique was 0.5 mg to 1 ng and 1 mg to 1 ng, respectively. The results obtained by biotechnology were similar to that by biosensor technique.
CONCLUSION
Biosensor technology was an accurate, convenient and rapid method for the determination of potency of endotoxin-neutralizing materials.
目的
探讨生物传感器技术在内毒素中和物质测定中的应用。
方法
将多粘菌素B（PMB）与一定浓度的内毒素混合后，分别采用生物传感器技术和鲎试剂法评估PMB对内毒素的中和率。比较两种方法的结果。
结果
生物传感器技术测定的PMB对内毒素的中和率为0.35微克比1纳克，而动态浊度法和显色鲎试剂法测定的中和率分别为0.5毫克比1纳克和1毫克比1纳克。生物技术法得到的结果与生物传感器技术法相似。
结论
生物传感器技术是一种准确、便捷、快速的内毒素中和物质效价测定方法。
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