基于鲎变形细胞溶解物蛋白酶反应监测的内毒素检测用石英晶体微天平传感器。

A quartz crystal microbalance sensor for endotoxin assay by monitoring limulus amebocyte lysate protease reaction.

机构信息

Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, 215163, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China.

Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, 215163, PR China.

出版信息

Anal Chim Acta. 2017 Apr 8;961:106-111. doi: 10.1016/j.aca.2017.01.014. Epub 2017 Jan 24.

DOI:10.1016/j.aca.2017.01.014

PMID:28224902

Abstract

Endotoxin is able to trigger strong innate immune responses by interacting with specific receptors on immune cells. Therefore, accurate and rapid detection of endotoxin is of primary importance. In this study, endotoxin induced viscosity variation of limulus amebocyte lysate (LAL) reagent is monitored by a quartz crystal microbalance (QCM) sensor with enhanced signal. Based on the analysis of the relationship between endotoxin concentration and QCM frequency shift with time, an effective sensing strategy is developed for endotoxin assay, which shows excellent sensitivity and specificity in the linear detection range from 0.005 to 10 EU mL. Moreover, this QCM sensor could be reused after a simple regeneration procedure. Therefore, it has potential practical utility for endotoxin determination in various applications.

摘要

内毒素能够通过与免疫细胞上的特定受体相互作用，引发强烈的固有免疫反应。因此，准确快速地检测内毒素至关重要。在本研究中，通过石英晶体微天平（QCM）传感器监测鲎变形细胞溶解物（LAL）试剂对内毒素诱导的粘度变化，从而增强信号。基于对内毒素浓度与 QCM 频率随时间变化关系的分析，建立了一种有效的内毒素检测传感策略，该策略在 0.005 至 10 EU mL 的线性检测范围内具有优异的灵敏度和特异性。此外，该 QCM 传感器在经过简单的再生处理后可重复使用。因此，它在各种应用中对内毒素的测定具有潜在的实用价值。