Stam Rianne, van Laar Teus-Jan, Wiegant Victor M
Department of Pharmacology and Anatomy, Rudolf Magnus Institute of Neuroscience, University Medical Center Utrecht, POB 85060, 3508 AB Utrecht, The Netherlands.
Physiol Behav. 2004 Mar;81(1):163-9. doi: 10.1016/j.physbeh.2004.01.015.
Luminal distention of the intestine can be aversive in humans and laboratory animals, and hypersensitivity to distention is found in functional gastrointestinal disorders. Current animal models either require anaesthesia or acute balloon intubation or use implanted balloons of irritant materials, for which the aversive quality of distention and physiological responses have not been well characterised. We report here that silicone balloon catheters implanted in the duodenum via the stomach have long patency without obvious tissue damage. Balloon inflation in freely moving rats caused passive avoidance learning and classic 'pain' postures, as well as graded cardiovascular responses which can be recorded telemetrically. The method should make long-lasting studies of pharmacological and environmental effects on visceral sensitivity more feasible.
肠腔扩张对人类和实验动物来说可能是令人厌恶的，并且在功能性胃肠疾病中发现对扩张存在超敏反应。目前的动物模型要么需要麻醉，要么需要急性球囊插管，要么使用刺激性材料制成的植入球囊，而对于这些模型，扩张的厌恶性质和生理反应尚未得到充分表征。我们在此报告，经胃将硅胶球囊导管植入十二指肠可实现长期通畅且无明显组织损伤。在自由活动的大鼠中进行球囊充气会引发被动回避学习和典型的“疼痛”姿势，以及可通过遥测记录的分级心血管反应。该方法应会使对内脏敏感性的药理和环境影响进行长期研究变得更加可行。
