互补的物理和遗传技术将纽蛋白（VCL）基因定位在10号染色体长臂上。

Complementary physical and genetic techniques map the vinculin (VCL) gene on chromosome 10q.

作者信息

Mulligan L M, Gardner E, Telenius H, Ponder B A

机构信息

Department of Pathology, University of Cambridge, United Kingdom.

出版信息

Genomics. 1992 Aug;13(4):1347-9. doi: 10.1016/0888-7543(92)90066-2.

DOI:10.1016/0888-7543(92)90066-2

PMID:1505973

Abstract

Vinculin is a cytoskeletal protein component of adherens type cell junctions. The gene had been mapped to 10q11.2-qter. We have used a combination of physical and genetic mapping techniques to refine this localization. Hybridization of the vinculin cDNA probe, HV1, to a human-rodent somatic hybrid panel initially suggested a position of either 10q11.2 or 10q22.1-10q23. Genetic recombination mapping in three-generation families with multiple endocrine neoplasia type 2 (MEN2) indicated a position distal to D10S22 (10q21.1) in 10q22.1-10q23. This was confirmed by hybridization of the vinculin cDNA to flow-sorted translocation derivative chromosomes containing the q21-qter portion of chromosome 10. We conclude that the vinculin locus maps in 10q22.1-q23, distal to D10S22.

摘要

纽蛋白是黏着连接型细胞连接的一种细胞骨架蛋白成分。该基因已被定位到10q11.2 - qter。我们使用了物理和遗传定位技术相结合的方法来优化这一定位。纽蛋白cDNA探针HV1与人 - 啮齿动物体细胞杂交板杂交，最初提示其位置在10q11.2或10q22.1 - 10q23。在患有2型多发性内分泌腺瘤（MEN2）的三代家族中进行的遗传重组定位表明，其位置在10q22.1 - 10q23中D10S22（10q21.1）的远端。通过将纽蛋白cDNA与包含10号染色体q21 - qter部分的流式分选易位衍生染色体杂交，这一点得到了证实。我们得出结论，纽蛋白基因座定位于10q22.1 - q23，在D10S22的远端。