肠炎沙门氏菌III型分泌相关ATP酶InvC的遗传分析确定了离散的功能域。
Genetic analysis of the Salmonella enterica type III secretion-associated ATPase InvC defines discrete functional domains.
作者信息
Akeda Yukihiro, Galán Jorge E
机构信息
Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, Connecticut 06536, USA.
出版信息
J Bacteriol. 2004 Apr;186(8):2402-12. doi: 10.1128/JB.186.8.2402-2412.2004.
Abstract
An essential component of all type III secretion systems is a highly conserved ATPase that shares significant amino acid sequence similarity to the beta subunit of the F(0)F(1) ATPases and is thought to provide the energy for the secretion process. We have performed a genetic and functional analysis of InvC, the ATPase associated with the Salmonella enterica type III secretion system encoded within its pathogenicity island 1. Through a mutagenesis analysis, we have identified amino acid residues that are essential for specific activities of InvC, such as nucleotide hydrolysis and membrane binding. This has allowed us to define discrete domains of InvC that are specifically associated with different essential activities of this protein.
摘要
所有III型分泌系统的一个重要组成部分是一种高度保守的ATP酶，它与F(0)F(1) ATP酶的β亚基具有显著的氨基酸序列相似性，被认为可为分泌过程提供能量。我们对InvC进行了遗传和功能分析，InvC是与肠道沙门氏菌III型分泌系统相关的ATP酶，编码于其1号致病岛中。通过诱变分析，我们确定了InvC特定活性（如核苷酸水解和膜结合）所必需的氨基酸残基。这使我们能够定义InvC的离散结构域，这些结构域与该蛋白的不同基本活性特异性相关。
相似文献
1
Genetic analysis of the Salmonella enterica type III secretion-associated ATPase InvC defines discrete functional domains.肠炎沙门氏菌III型分泌相关ATP酶InvC的遗传分析确定了离散的功能域。
J Bacteriol. 2004 Apr;186(8):2402-12. doi: 10.1128/JB.186.8.2402-2412.2004.
2
Molecular and functional characterization of the Salmonella typhimurium invasion genes invB and invC: homology of InvC to the F0F1 ATPase family of proteins.鼠伤寒沙门氏菌侵袭基因invB和invC的分子与功能特性：InvC与F0F1 ATP酶蛋白家族的同源性
J Bacteriol. 1994 Aug;176(15):4501-10. doi: 10.1128/jb.176.15.4501-4510.1994.
3
Structural Features Reminiscent of ATP-Driven Protein Translocases Are Essential for the Function of a Type III Secretion-Associated ATPase.与ATP驱动的蛋白质转运酶相似的结构特征对于III型分泌相关ATP酶的功能至关重要。
J Bacteriol. 2015 Sep;197(18):3007-14. doi: 10.1128/JB.00434-15. Epub 2015 Jul 13.
4
Salmonella pathogenicity island 1 (SPI-1) type III secretion of SopD involves N- and C-terminal signals and direct binding to the InvC ATPase.沙门氏菌致病性岛 1（SPI-1）III 型分泌 SopD 需要 N 端和 C 端信号以及与 InvC ATP 酶的直接结合。
Microbiology (Reading). 2010 Jun;156(Pt 6):1805-1814. doi: 10.1099/mic.0.038117-0. Epub 2010 Feb 25.
5
Disruption of type III secretion in Salmonella enterica serovar Typhimurium by external guide sequences.外部引导序列对鼠伤寒沙门氏菌III型分泌的破坏
Nucleic Acids Res. 2004 Feb 3;32(2):848-54. doi: 10.1093/nar/gkh219. Print 2004.
6
Controlled Activity of the Invasion-Associated Injectisome Reveals Its Intracellular Role in the Cytosolic Population.控制侵袭相关注入器的活性揭示了其在细胞质群体中的细胞内作用。
mBio. 2017 Dec 5;8(6):e01931-17. doi: 10.1128/mBio.01931-17.
7
Impact of Salmonella Typhimurium DT104 virulence factors invC and sseD on the onset, clinical course, colonization patterns and immune response of porcine salmonellosis.鼠伤寒沙门氏菌DT104毒力因子invC和sseD对猪沙门氏菌病的发病、临床病程、定植模式及免疫反应的影响
Vet Microbiol. 2007 Oct 6;124(3-4):274-85. doi: 10.1016/j.vetmic.2007.04.032. Epub 2007 Apr 27.
8
Insight into the flagella type III export revealed by the complex structure of the type III ATPase and its regulator.III型ATP酶及其调节因子的复杂结构揭示了鞭毛III型输出的奥秘。
Proc Natl Acad Sci U S A. 2016 Mar 29;113(13):3633-8. doi: 10.1073/pnas.1524025113. Epub 2016 Mar 16.
9
Common architecture of the flagellar type III protein export apparatus and F- and V-type ATPases.鞭毛型 III 蛋白分泌装置和 F-型与 V-型 ATP 酶的通用结构。
Nat Struct Mol Biol. 2011 Mar;18(3):277-82. doi: 10.1038/nsmb.1977. Epub 2011 Jan 30.
10
Evolutionary links between FliH/YscL-like proteins from bacterial type III secretion systems and second-stalk components of the FoF1 and vacuolar ATPases.细菌III型分泌系统中FliH/YscL样蛋白与FoF1和液泡ATP酶的第二柄部组件之间的进化联系。
Protein Sci. 2006 Apr;15(4):935-41. doi: 10.1110/ps.051958806. Epub 2006 Mar 7.
引用本文的文献
1
Hyperpiliation, not loss of pilus retraction, reduces pathogenicity.毛发过多而非菌毛收缩功能丧失会降低致病性。
Microbiol Spectr. 2025 Apr;13(4):e0255824. doi: 10.1128/spectrum.02558-24. Epub 2025 Feb 25.
2
Purpurin suppresses Salmonella invasion of host cells by reducing the secretion of T3SS-1 effector proteins.紫红素通过减少三型分泌系统1效应蛋白的分泌来抑制沙门氏菌对宿主细胞的侵袭。
Sci Rep. 2025 Feb 6;15(1):4507. doi: 10.1038/s41598-025-86822-1.
3
Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.革兰氏阴性菌中三部分外排和 1 型分泌系统的结构、组装和功能。
Chem Rev. 2021 May 12;121(9):5479-5596. doi: 10.1021/acs.chemrev.1c00055. Epub 2021 Apr 28.
4
Novel Noncompetitive Type Three Secretion System ATPase Inhibitors Shut Down Effector Secretion.新型非竞争性 III 型分泌系统 ATP 酶抑制剂可阻断效应物分泌。
Biochemistry. 2020 Jul 21;59(28):2667-2678. doi: 10.1021/acs.biochem.0c00431. Epub 2020 Jun 30.
5
Dominant negative effects by inactive Spa47 mutants inhibit T3SS function and Shigella virulence.无效 Spa47 突变体的显性负效应抑制 T3SS 功能和志贺氏菌毒力。
PLoS One. 2020 Jan 24;15(1):e0228227. doi: 10.1371/journal.pone.0228227. eCollection 2020.
6
Structural analysis of ligand-bound states of the Salmonella type III secretion system ATPase InvC.沙门氏菌 III 型分泌系统 ATP 酶 InvC 配体结合态的结构分析。
Protein Sci. 2019 Oct;28(10):1888-1901. doi: 10.1002/pro.3704. Epub 2019 Aug 24.
7
Interfacial amino acids support Spa47 oligomerization and shigella type three secretion system activation.界面氨基酸支持 Spa47 寡聚化和志贺氏菌 III 型分泌系统的激活。
Proteins. 2019 Nov;87(11):931-942. doi: 10.1002/prot.25754. Epub 2019 Jun 21.
8
Cryo-EM structure of the homohexameric T3SS ATPase-central stalk complex reveals rotary ATPase-like asymmetry.同源六聚体 T3SS ATP 酶-中心管复合物的冷冻电镜结构揭示了旋转 ATP 酶样不对称性。
Nat Commun. 2019 Feb 7;10(1):626. doi: 10.1038/s41467-019-08477-7.
9
Role of SpaO in the assembly of the sorting platform of a Salmonella type III secretion system.SpaO 在沙门氏菌 III 型分泌系统分选平台组装中的作用。
PLoS Pathog. 2019 Jan 22;15(1):e1007565. doi: 10.1371/journal.ppat.1007565. eCollection 2019 Jan.
10
Shutting Down Shigella Secretion: Characterizing Small Molecule Type Three Secretion System ATPase Inhibitors.阻断志贺氏菌分泌：小分子三型分泌系统ATP酶抑制剂的特性研究
Biochemistry. 2018 Dec 18;57(50):6906-6916. doi: 10.1021/acs.biochem.8b01077. Epub 2018 Dec 5.
本文引用的文献
1
MxiK and MxiN interact with the Spa47 ATPase and are required for transit of the needle components MxiH and MxiI, but not of Ipa proteins, through the type III secretion apparatus of Shigella flexneri.MxiK和MxiN与Spa47 ATP酶相互作用，是弗氏志贺菌III型分泌系统转运针状组件MxiH和MxiI（而非Ipa蛋白）所必需的。
Mol Microbiol. 2003 Aug;49(3):755-67. doi: 10.1046/j.1365-2958.2003.03590.x.
2
SWISS-MODEL: An automated protein homology-modeling server.SWISS-MODEL：一个自动化的蛋白质同源建模服务器。
Nucleic Acids Res. 2003 Jul 1;31(13):3381-5. doi: 10.1093/nar/gkg520.
3
Oligomerization and activation of the FliI ATPase central to bacterial flagellum assembly.FliI ATP酶的寡聚化与激活对于细菌鞭毛组装至关重要。
Mol Microbiol. 2003 Jun;48(5):1349-55. doi: 10.1046/j.1365-2958.2003.03506.x.
4
Type III protein translocase: HrcN is a peripheral ATPase that is activated by oligomerization.III型蛋白质转运酶：HrcN是一种外周ATP酶，通过寡聚化被激活。
J Biol Chem. 2003 Jul 11;278(28):25816-24. doi: 10.1074/jbc.M301903200. Epub 2003 May 6.
5
How bacteria assemble flagella.细菌如何组装鞭毛。
Annu Rev Microbiol. 2003;57:77-100. doi: 10.1146/annurev.micro.57.030502.090832. Epub 2003 May 1.
6
VirB11 ATPases are dynamic hexameric assemblies: new insights into bacterial type IV secretion.VirB11 ATP酶是动态六聚体组装体：对细菌IV型分泌的新见解。
EMBO J. 2003 May 1;22(9):1969-80. doi: 10.1093/emboj/cdg223.
7
Salmonella type III secretion-associated protein InvE controls translocation of effector proteins into host cells.鼠伤寒沙门氏菌III型分泌相关蛋白InvE控制效应蛋白向宿主细胞的转运。
J Bacteriol. 2002 Sep;184(17):4699-708. doi: 10.1128/JB.184.17.4699-4708.2002.
8
Intrinsic membrane targeting of the flagellar export ATPase FliI: interaction with acidic phospholipids and FliH.鞭毛输出ATP酶FliI的内在膜靶向作用：与酸性磷脂和FliH的相互作用。
J Mol Biol. 2002 May 10;318(4):941-50. doi: 10.1016/S0022-2836(02)00172-9.
9
Salmonella interactions with host cells: type III secretion at work.沙门氏菌与宿主细胞的相互作用：发挥作用的III型分泌系统
Annu Rev Cell Dev Biol. 2001;17:53-86. doi: 10.1146/annurev.cellbio.17.1.53.
10
The bacterial conjugation protein TrwB resembles ring helicases and F1-ATPase.细菌接合蛋白TrwB类似于环状解旋酶和F1 - ATP酶。
Nature. 2001 Feb 1;409(6820):637-41. doi: 10.1038/35054586.
Zotero 插件