McKinney Jeffrey S, Zhang Haifeng, Kubori Tomoko, Galán Jorge E, Altman Sidney
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA.
Nucleic Acids Res. 2004 Feb 3;32(2):848-54. doi: 10.1093/nar/gkh219. Print 2004.
The type III secretion system involved in Salmonella enterica serovar Typhimurium invasion of host cells has been disrupted using inducibly expressed oligonucleotide external guide sequences (EGSs) complementary to invB or invC mRNA. These EGSs direct single site cleavage in these mRNAs by endogenous RNase P, and their expression in Salmonella results in invC mRNA and InvC protein depletion, decreased type III secretion and interference with host cell invasion. Comparison of these effects with those from studies of Salmonella invB and invC mutants suggests that invB EGSs have polar effects on invC mRNA.
利用可诱导表达的与invB或invC mRNA互补的寡核苷酸外部引导序列(EGS),破坏了参与鼠伤寒沙门氏菌侵袭宿主细胞的III型分泌系统。这些EGS通过内源性核糖核酸酶P指导这些mRNA中的单一位点切割,它们在沙门氏菌中的表达导致invC mRNA和InvC蛋白耗竭,III型分泌减少,并干扰宿主细胞侵袭。将这些效应与沙门氏菌invB和invC突变体研究的效应进行比较表明,invB EGS对invC mRNA具有极性效应。