艰难梭菌IStron CdISt1：一种编码两种完整转座酶样蛋白的变体的发现
Clostridium difficile IStron CdISt1: discovery of a variant encoding two complete transposase-like proteins.
作者信息
Hasselmayer Oliver, Braun Veit, Nitsche Claudia, Moos Michael, Rupnik Maja, von Eichel-Streiber Christoph
机构信息
Institute of Medical Microbiology and Hygiene, Johannes Gutenberg University of Mainz, D-55101 Mainz, Germany.
出版信息
J Bacteriol. 2004 Apr;186(8):2508-10. doi: 10.1128/JB.186.8.2508-2510.2004.
Abstract
Screening a Clostridium difficile strain collection for the chimeric element CdISt1, we identified two additional variants, designated CdISt1-0 and CdISt1-III. In in vitro assays, we could prove the self-splicing ribozyme activity of these variants. Structural comparison of all known CdISt1 variants led us to define four types of IStrons that we designated CdISt1-0 through CdISt1-III. Since CdISt1-0 encodes two complete transposase-like proteins (TlpA and TlpB), we suggest that it represents the original genetic element, hypothesized before to have originated by fusion of a group I intron and an insertion sequence element.
摘要
在艰难梭菌菌株库中筛选嵌合元件CdISt1时，我们鉴定出另外两个变体，命名为CdISt1-0和CdISt1-III。在体外试验中，我们能够证明这些变体的自我剪接核酶活性。对所有已知CdISt1变体的结构比较使我们定义了四种内含子类型，我们将其命名为CdISt1-0至CdISt1-III。由于CdISt1-0编码两种完整的转座酶样蛋白（TlpA和TlpB），我们认为它代表了原始遗传元件，之前推测它是由I组内含子和插入序列元件融合产生的。
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FEMS Microbiol Lett. 2001 May 1;198(2):171-6. doi: 10.1111/j.1574-6968.2001.tb10638.x.
2
Barriers to intron promiscuity in bacteria.细菌中内含子乱交的障碍。
J Bacteriol. 2000 Oct;182(19):5281-9. doi: 10.1128/JB.182.19.5281-5289.2000.
3
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4
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Gene. 1998 Nov 26;223(1-2):175-86. doi: 10.1016/s0378-1119(98)00164-4.
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Microbiol Mol Biol Rev. 1998 Sep;62(3):725-74. doi: 10.1128/MMBR.62.3.725-774.1998.
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