Nath P, Bhakta M, Mitra K
Department of Zoology, Visva-Bharati University, Santiniketan, India.
Indian J Exp Biol. 1992 Jun;30(6):464-9.
Vitellogenin (Vg) synthesis was induced in the male and non-vitellogenic female Rohu, the Indian major carp, by estradiol-17 beta(E2) where effect was more in female. A crude preparation of Vg was isolated in the second peak after gel filtration on Ultrogel AcA 34 from the sera of vitellogenic female Rohu and E2-treated male and female Rohu. Estimation of alkali-labile phosphorus was shown to be used as an index of Vg. Native-PAGE analysis has revealed the presence of two forms of Vg (Vg1: 430,000 dalton and Vg2:240,000 dalton) in Vg fraction obtained after gel filtration as well as in the sera of E2-treated male and female Rohu. Immunological cross-reaction studies between antiserum to yolk protein and Vg fractions as well as the sera from E2-treated male and female Rohu further indicates the presence of two precipitin lines (not clearly visible as the two lines fused to form a thick line) suggesting the occurrence of two forms of Vg in the Rohu.
在雄性和未发生卵黄生成的雌性印度主要鲤鱼——露斯塔野鲮中，17β-雌二醇（E2）诱导了卵黄蛋白原（Vg）的合成，其中对雌性的影响更大。在经凝胶过滤的Ultrogel AcA 34上，从发生卵黄生成的雌性露斯塔野鲮以及经E2处理的雄性和雌性露斯塔野鲮的血清中，在第二个峰中分离出了Vg的粗制品。碱不稳定磷的测定表明可将其用作Vg的指标。天然聚丙烯酰胺凝胶电泳分析显示，在凝胶过滤后获得的Vg组分以及经E2处理的雄性和雌性露斯塔野鲮的血清中，存在两种形式的Vg（Vg1：430,000道尔顿和Vg2：240,000道尔顿）。卵黄蛋白抗血清与Vg组分以及经E2处理的雄性和雌性露斯塔野鲮血清之间的免疫交叉反应研究进一步表明存在两条沉淀线（由于两条线融合形成一条粗线而不太清晰可见），这表明露斯塔野鲮中存在两种形式的Vg。
