James R, Sampath K, Ponmani K P
Department of Zoology, V.O. Chidambaram College, Tuticorin, India.
Indian J Exp Biol. 1992 Jun;30(6):496-9.
Effects of sublethal doses of metal (Cu, Cd, Zn) mixtures on the activities of key respiratory enzymes (succinate dehydrogenase, SDH and glyceraldehyde dehydrogenase, GDH) and their recovery following withdrawal of treatments were studied in the freshwater fish O. mossambicus. On the basis of 96 hr LC50 Cu was highly toxic followed by Zn and Cd, and the trimetal combination (Cu+Zn+Cd) was extremely toxic than any other combination; combination of Zn+Cd was least toxic. A significant gradual decrease in SDH with a concomitant increase in GDH activity observed in liver, brain, muscle and gill of animals exposed to metal suggest a metabolic shift from aerobiosis to anaerobiosis due to metal action. Exposed individuals when transferred to metal impoverished water showed an improvement in SDH activity and decline in GDH activity suggesting slow reversal to aerobic metabolism. O. mossambicus needs more time for complete recovery.
研究了亚致死剂量的金属（铜、镉、锌）混合物对淡水鱼莫桑比克罗非鱼关键呼吸酶（琥珀酸脱氢酶，SDH和甘油醛脱氢酶，GDH）活性的影响以及停止处理后它们的恢复情况。根据96小时半数致死浓度（LC50），铜的毒性最高，其次是锌和镉，三金属组合（铜+锌+镉）比任何其他组合的毒性都大得多；锌+镉组合的毒性最小。在暴露于金属的动物的肝脏、大脑、肌肉和鳃中观察到，SDH显著逐渐降低，同时GDH活性增加，这表明由于金属作用，代谢从需氧代谢转变为厌氧代谢。当将暴露的个体转移到贫金属水中时，SDH活性有所改善，GDH活性下降，这表明向有氧代谢的缓慢逆转。莫桑比克罗非鱼需要更多时间才能完全恢复。
