Mazumder D N, Santra A, Dutta G, Ghosh N, Chowdhury M K
Department of Gastroenterology, Institute of Post-Graduate Medical Education and Research, Calcutta.
Indian J Gastroenterol. 1992 Jul;11(3):117-20.
To ascertain the role of excretory and secretory (ES) products of Ascaris lumbricoides in liver damage.
The ES products of A lumbricoides were collected in vitro and their SDS-PAGE analysis was done. Feeding and subcutaneous injection of ES products were done in hamsters. Estimation of serum proteins, alkaline phosphatase and alanine aminotransferase and histology of liver were carried out. Control animal experiments were done concurrently.
The ES products of A lumbricoides contained several proteins ranging in molecular weight from 14 to 205 Kd. Prolonged feeding of ES products caused elevation of ALT and amyloid deposition in the liver, whereas short term feeding or subcutaneous challenge caused focal cell necrosis and granuloma formation in the liver.
ES products of A lumbricoides can produce liver damage.
确定蛔虫排泄分泌（ES）产物在肝脏损伤中的作用。
体外收集蛔虫的ES产物并进行SDS-PAGE分析。对仓鼠进行ES产物灌胃和皮下注射。检测血清蛋白、碱性磷酸酶和丙氨酸氨基转移酶，并进行肝脏组织学检查。同时进行对照动物实验。
蛔虫的ES产物含有几种分子量在14至205 Kd之间的蛋白质。长期灌胃ES产物会导致谷丙转氨酶升高和肝脏淀粉样沉积，而短期灌胃或皮下注射则会导致肝脏局灶性细胞坏死和肉芽肿形成。
蛔虫的ES产物可造成肝脏损伤。
