Kuta Elzbieta, Bohanec Borut, Dubas Ewa, Vizintin Liliana, Przywara Leslaw
Department of Plant Cytology and Embryology, Jagiellonian University, Cracow, Poland.
Genome. 2004 Apr;47(2):246-56. doi: 10.1139/g03-121.
Chromosomes and nuclear DNA amount were analyzed in leaf tissues of Luzula nivea, Luzula luzuloides, and Luzula multiflora. Intra- and interspecific karyological variability was stated. Chromosome numbers in diploids ranged 2n = 8-24 in L. nivea and L. luzuloides and 2n = 12-84 in hexaploid L. multiflora. Karyological variability resulted mainly from chromosome fission (agmatoploidy) and aneusomaty; chromosome fusion (symploidy) and polyploidy were also involved. Flow cytometric determination of nuclear genome size using propidium iodide staining gave values of 1.584 pg in L. luzuloides, 1.566 pg in L. nivea, and 3.034 pg in L. multiflora. Variability in relative nuclear genome size within species was measured by 4',6-diamidino-2-phenylindole staining. In contrast with previous reports, variability was fairly small and ranged from 1.796 to 1.864 pg in L. luzuloides, from 1.783 to 1.847 pg and from 1.737 to 1.808 pg in two populations (S and F) of L. nivea, respectively, and from 3.125 to 3.271 pg in L. multiflora. An intraplant (interleaf) genome size variation was also observed and its possible causes are discussed.
对雪白灯心草、类灯心草和多花灯心草的叶片组织进行了染色体和核DNA含量分析。阐述了种内和种间的核型变异。二倍体的染色体数在雪白灯心草和类灯心草中为2n = 8 - 24，在六倍体多花灯心草中为2n = 12 - 84。核型变异主要源于染色体裂变（染色体断裂加倍）和异数性；染色体融合（整倍体）和多倍体也有涉及。使用碘化丙啶染色通过流式细胞术测定核基因组大小，结果显示类灯心草为1.584 pg，雪白灯心草为1.566 pg，多花灯心草为3.034 pg。通过4',6 - 二脒基 - 2 - 苯基吲哚染色测量了物种内相对核基因组大小的变异。与之前的报道相反，变异相当小，类灯心草的变异范围为1.796至1.864 pg，雪白灯心草的两个种群（S和F）分别为1.783至1.847 pg和1.737至1.808 pg，多花灯心草为3.125至3.271 pg。还观察到了植株内（叶间）基因组大小的变异，并讨论了其可能的原因。
