Woram R A, McGowan C, Stout J A, Gharbi K, Ferguson M M, Hoyheim B, Davidson E A, Davidson W S, Rexroad C, Danzmann R G
Department of Zoology, University of Guelph, ON, Canada.
Genome. 2004 Apr;47(2):304-15. doi: 10.1139/g03-127.
We constructed a genetic linkage map for Arctic char (Salvelinus alpinus) using two backcrosses between genetically divergent strains. Forty-six linkage groups (expected = 39-41) and 19 homeologous affinities (expected = 25) were identified using 184 microsatellites, 129 amplified fragment length polymorphisms (AFLPs), 13 type I gene markers, and one phenotypic marker, SEX. Twenty-six markers remain unlinked. Female map distance (9.92 Morgans) was substantially higher than male map distance (3.90 Morgans) based on the most complete parental information (i.e., the F1 hybrids). Female recombination rates were often significantly higher than those of males across all pairwise comparisons within homologous chromosomal segments (average female to male ratios within families was 1.69:1). The female hybrid parent had significantly higher recombination rates than the pure strain female parent. Segregation distortion was detected in four linkage groups (4, 8, 13, 20) for both families. In family 3, only the largest fish were sampled for genotyping, suggesting that segregation distortion may represent regions possessing influences on growth. In family 2, almost all cases showing segregation distortion involved markers in the female hybrid parent.
我们利用两个基因差异较大的品系之间的回交构建了北极红点鲑（Salvelinus alpinus）的遗传连锁图谱。使用184个微卫星、129个扩增片段长度多态性（AFLP）、13个I型基因标记和一个表型标记SEX，鉴定出46个连锁群（预期为39 - 41个）和19个同源亲和力（预期为25个）。26个标记仍未连锁。基于最完整的亲本信息（即F1杂种），雌性图谱距离（9.92摩根）显著高于雄性图谱距离（3.90摩根）。在同源染色体片段内的所有成对比较中，雌性重组率通常显著高于雄性（家系内雌性与雄性的平均比率为1.69:1）。雌性杂种亲本的重组率显著高于纯系雌性亲本。在两个家系的四个连锁群（4、8、13、20）中检测到分离畸变。在第3家系中，仅对最大的鱼进行了基因分型采样，这表明分离畸变可能代表了对生长有影响的区域。在第2家系中，几乎所有显示分离畸变的情况都涉及雌性杂种亲本中的标记。
