Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China.
National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China.
Mar Biotechnol (NY). 2017 Oct;19(5):488-496. doi: 10.1007/s10126-017-9768-2. Epub 2017 Jul 19.
The razor clam (Sinonovacula constricta) is an important aquaculture species, for which a high-density genetic linkage map would play an important role in marker-assisted selection (MAS). In this study, we constructed a high-density genetic map and detected quantitative trait loci (QTLs) for Sinonovacula constricta with an F cross population by using the specific locus amplified fragment sequencing (SLAF-seq) method. A total of 315,553 SLAF markers out of 467.71 Mreads were developed. The final linkage map was composed of 7516 SLAFs (156.60-fold in the parents and 20.80-fold in each F population on average). The total distance of the linkage map was 2383.85 cM, covering 19 linkage groups with an average inter-marker distance of 0.32 cM. The proportion of gaps less than 5.0 cM was on average 96.90%. A total of 16 suggestive QTLs for five growth-related traits (five QTLs for shell height, six QTLs for shell length, three QTLs for shell width, one QTL for total body weight, and one QTL for soft body weight) were identified. These QTLs were distributed on five linkage groups, and the regions showed overlapping on LG9 and LG13. In conclusion, the high-density genetic map and QTLs for S. constricta provide a valuable genetic resource and a basis for MAS.
刀额新对虾（Sinonovacula constricta）是一种重要的水产养殖品种，高密度遗传图谱将在标记辅助选择（MAS）中发挥重要作用。本研究利用特异区域扩增片段测序（SLAF-seq）方法，以 F1 杂交群体构建了高密度遗传图谱，并检测了刀额新对虾的数量性状位点（QTLs）。共开发了 315553 个 SLAF 标记，平均每个亲本和每个 F1 群体的有效标记数分别为 467.71Mreads 的 156.60 倍和 20.80 倍。最终的连锁图谱由 7516 个 SLAF 标记组成（连锁群），总长度为 2383.85cM，平均标记间距离为 0.32cM。连锁群中小于 5.0cM 的间隔比例平均为 96.90%。共检测到与五个生长相关性状（壳高五个 QTL、壳长六个 QTL、壳宽三个 QTL、总重一个 QTL、软体重一个 QTL）相关的 16 个候选 QTLs。这些 QTL 分布在五个连锁群上，LG9 和 LG13 上的区域存在重叠。综上所述，高密度遗传图谱和刀额新对虾的 QTLs 为 MAS 提供了有价值的遗传资源和基础。
